A transposon in the vacuolar sorting receptor gene TaVSR1-B promoter region is associated with wheat root depth at booting stage.
A transposon in the vacuolar sorting receptor gene TaVSR1-B promoter region is associated with wheat root depth at booting stage.
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DOI：
10.1111/pbi.13564
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发表时间：
2021-07
影响因子：
13.8
通讯作者：
Jing R
中科院分区：
文献类型：
--
作者：
Wang J;Li L;Li C;Yang X;Xue Y;Zhu Z;Mao X;Jing R
根深是根构型的重要组成部分，在作物生长发育、产量决定和非生物胁迫耐受性等方面具有重要作用，但其遗传基础尚不清楚。本研究以323个小麦（Triticum aestivum L.）对100份小麦种质的根深变异进行评估，并用小麦660 K SNP阵列进行基因分型。GWAS（全基因组关联研究）检测到TaVSR 1-B基因启动子中的125 bp微型反向重复转座因子（MITE）与孕穗期根深之间存在显著关联。我们发现MITE通过DNA甲基化和H3 K27三甲基化抑制TaVSR 1-B表达。TaVSR 1-B在根生长中的作用通过该基因在转基因小麦、水稻和tavsr 1 TILLING突变体中的表达改变来验证。TaVSR 1 B表达增加使根伸长区变短，分化区变长，导致根变深。这项工作为小麦根深变异的遗传基础提供了新的见解，并为小麦根构型的遗传改良提供了一个有前途的目标。
Root depth, as an important component of root architecture, plays a significant role in growth, grain yield determination and abiotic stress tolerance in crop plants, but its genetic basis remains poorly elucidated. In this study, a panel composed of 323 wheat (Triticum aestivum L.) accessions was assessed for variation in root depth and genotyped with the Wheat 660K SNP Array. GWAS (genome‐wide association study) detected significant association between a 125 bp miniature inverted‐repeat transposable element (MITE) in the promoter of the TaVSR1‐B gene with root depth at the booting stage. We showed that the MITE repressed TaVSR1‐B expression by DNA methylation and H3K27 tri‐methylation. The roles of TaVSR1‐B in root growth were verified by altered expression of the gene in transgenic wheat, rice and a tavsr1 TILLING mutant. Increased TaVSR1‐B expression made the root elongation zone shorter and the differentiation zone longer, leading to deeper root. This work provides novel insight into the genetic basis of variation in root depth and a promising target for genetic improvement of root architecture in wheat.
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影响因子：
27.5
作者：
Pang, Yunlong;Liu, Chunxia;Liu, Shubing
通讯作者：
Liu, Shubing
影响因子：
4.2
作者：
Li, Long;Peng, Zhi;Jing, Ruilian
通讯作者：
Jing, Ruilian
影响因子：
11.4
作者：
Promchuea, Sujittra;Zhu, Yujuan;Gong, Zhizhong
通讯作者：
Gong, Zhizhong
DOI：
10.1073/pnas.1217133110
发表时间：
2013-05-14
影响因子：
11.1
作者：
Cavanagh, Colin R.;Chao, Shiaoman;Akhunov, Eduard
通讯作者：
Akhunov, Eduard
影响因子：
12.3
作者：
Gardiner LJ;Quinton-Tulloch M;Olohan L;Price J;Hall N;Hall A
通讯作者：
Hall A