Newcastle Disease Virus V Protein Promotes Viral Replication in HeLa Cells through the Activation of MEK/ERK Signaling.
Newcastle Disease Virus V Protein Promotes Viral Replication in HeLa Cells through the Activation of MEK/ERK Signaling.
复制标题
新城疫病毒 V 蛋白通过激活 MEK/ERK 信号传导促进 HeLa 细胞中的病毒复制
DOI：
10.3390/v10090489
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发表时间：
2018-09-12
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Chu Z;Ma J;Wang C;Lu K;Li X;Liu H;Wang X;Xiao S;Yang Z
Newcastle disease virus (NDV) can infect a wide range of domestic and wild bird species. The non-structural V protein of NDV plays an important role in antagonizing innate host defenses to facilitate viral replication. However, there is a lack of knowledge related to the mechanisms through which the V protein regulates viral replication. The extracellular signal-regulated kinase (ERK) signaling pathway in the host is involved in a variety of functions and is activated by several stimuli, including viral replication. In this study, we show that both the lentogenic strain, La Sota, and the velogenic strain, F48E9, of NDV activate the mitogen-activated protein kinase (MEK)/ERK signaling pathway. The pharmacological inhibition of ERK1/2 phosphorylation using the highly selective inhibitors U0126 and SCH772984 resulted in the reduced levels of NDV RNA in cells and virus titers in the cell supernatant, which established an important role for the MEK/ERK signaling pathway in NDV replication. Moreover, the overexpression of the V protein in HeLa cells increased the phosphorylation of ERK1/2 and induced the transcriptional changes in the genes downstream of the MEK/ERK signaling pathway. Taken together, our results demonstrate that the V protein is involved in the ERK signaling pathway-mediated promotion of NDV replication and thus, can be investigated as a potential antiviral target.
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影响因子：
5.2
作者：
Morris MA;Laverick L;Wei W;Davis AM;O'Neill S;Wood L;Wright J;Dawson CW;Young LS
通讯作者：
Young LS
影响因子：
4.8
作者：
Marjuki, Henju;Alam, Mohammad I.;Pleschka, Stephan
通讯作者：
Pleschka, Stephan
影响因子：
5.4
作者：
Moser, Lindsey A.;Schultz-Cherry, Stacey
通讯作者：
Schultz-Cherry, Stacey
DOI：
10.3390/v10040199
发表时间：
2018-04-17
期刊：
Viruses
影响因子：
--
作者：
Brunetti JE;Foscaldi S;Quintana VM;Scolaro LA;López N;Castilla V
通讯作者：
Castilla V
影响因子：
3.8
作者：
Dai, Mantuan;Feng, Min;Cao, Weisheng
通讯作者：
Cao, Weisheng