Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication.
Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication.
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大豆苷是一种抗氧化剂异黄酮类化合物，可降低血液中的酒精含量并缩短乙醇中毒引起的睡眠时间。
DOI：
10.1111/j.1530-0277.1994.tb01448.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wang,GF
中科院分区：
文献类型：
--
作者：
Xie,CI;Lin,RC;Antony,V;Lumeng,L;Li,TK;Mai,K;Liu,C;Wang,QD;Zhao,ZH;Wang,GF
据报道，从一种可食用的藤蔓植物葛根中提取的提取物在减轻酒精中毒方面是有效的。在这项研究中，我们已经测试了这种植物提取物的主要成分之一，大豆苷的功效。禁食大鼠灌胃乙醇（40%，3g/kg体重）后，血中乙醇浓度（BAC）在30 min达高峰，为1.77 ± 0.14mg/ml（平均值±标准差，n =6）。如果将大豆苷（30 mg/kg）与乙醇溶液混合并灌胃给动物，则发现BAC在摄入乙醇后90 min达到峰值，仅达到1.20 ± 0.30mg/ml（n = 6）（与对照组相比p < 0.05）。大豆苷延迟和降低酒精摄入后的BAC水平峰值的能力也观察到在喂养的动物，在禁食和喂养的大鼠给予酒精没有大豆苷，BAC迅速下降后，在30分钟达到峰值。相比之下，BAC水平下降得更慢，如果大豆苷也喂给动物。大豆苷表现出慢性效应。在乙醇攻击前7天喂食大豆苷的大鼠，但在攻击当天不喂食大豆苷，也产生较低和较晚的BAC峰值水平。有趣的是，大豆苷，无论是喂给大鼠一次或慢性7天，没有显着改变无论是乙醇脱氢酶或线粒体乙醛脱氢酶在肝脏中的活动。进一步的实验表明，大豆苷缩短睡眠时间的大鼠接受乙醇灌胃（7 g/ kg），而不是腹腔注射（2 g/kg）。为了测试大豆苷是否延迟胃排空，将聚乙二醇与乙醇混合并喂给大鼠。结果发现，灌胃后30分钟，更多的乙醇和[14 C]聚乙二醇留在胃中，如果大鼠也给予大豆苷。因为大豆苷是具有强抗氧化活性的大豆糖苷，所以另外两种抗氧化剂（即，维生素E和硫辛酸）。与大豆苷类似，这两种抗氧化剂也延迟和抑制BAC峰值，以及缩短酒精摄入引起的睡眠时间。我们的结论是：（1）大豆苷可有效对抗酒精中毒;（2）大豆苷对BAC的抑制主要是由于胃排空延迟，而不是由于肝酶加速循环中乙醇的清除;（3）大豆苷的作用可能部分是由于其抗氧化活性。
The extract from an edible vine,Pueraha lebatahas been reported to be efficacious in lessening alcohol intoxication. In this study, we have tested the efficacy of one of the major components, daidzin, from this plant extract. When ethanol (40% solution, 3 g/kg body weight) was given to fasted rats intragastrically, blood alcohol concentration (BAC) peaked at 30 min after alcohol ingestion and reached 1.77 ± 0.14 mg/ml (mean values ±sd,n =6). If daidzin (30 mg/kg) was mixed with the ethanol solution and given to animals intragastrically, BAC was found to peak at 90 min after alcohol ingestion and reached only 1.20 ± 0.30 mg/ml (n = 6) (p < 0.05 vs. controls). The ability of daidzin to delay and decrease peak BAC level after ethanol ingestion was also observed in fed animals, in both fasted and fed rats given alcohol without daidzin, BAC quickly declined after reaching its peak at 30 min. By contrast, BAC levels receded more slowly if daidzin was also fed to the animals. Daidzin showed a chronic effect. Rats fed daidzin for 7 days before ethanol challenge, but not on the day of challenge, also produced lower and later peak BAC levels. Interestingly, daidzin, whether fed to rats only once or chronically for 7 days, did not significantly alter activities of either alcohol dehydrogenase or mitochondrial aldehyde dehydrogenase in the liver. Further experiments demonstrated that daidzin shortened sleep time for rats receiving ethanol intragastrically (7 g/ kg) but not intraperitoneally (2 g/kg). To test whether daidzin delayed stomach‐emptying, [Clpolyethylene glycol was mixed with ethanol and fed to rats. It was found that, 30 min after intragastric feeding, more ethanol and [14C]polyethylene glycol remained in the stomach if rats were also given daidzin. Because daidzin is an isoflavonoid glucoside that possesses strong antioxidant activity, two other antioxidants (i.e., vitamin E and thioctic acid) were tested. Similar to daidzin, these two antioxidants also delayed and suppressed peak BAC, as well as shortened sleep time induced by alcohol ingestion. We conclude that: (1) daidzin is effective in countering alcohol intoxication; (2) suppression of BAC by daidzin is due mainly to delay of stomach‐emptying, but not to accelerated clearance of ethanol in circulation by liver enzymes; and (3) the effect of daidzin may in part be due to its antioxidant activity.
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影响因子：
6.1
作者：
Caballeria,J;Baraona,E;Lieber,CS
通讯作者：
Lieber,CS
离体大鼠肝细胞中乙醇代谢率。
DOI：
--
发表时间：
1977
期刊：
Alcoholism: Clinical and Experimental Research
影响因子：
--
作者：
K. E. Crow;N. Cornell;R. Veech
通讯作者：
R. Veech
[3] 动物组织细胞成分的分离
DOI：
--
发表时间：
1955
期刊：
影响因子：
--
作者：
G. H. Hogeboom
通讯作者：
G. H. Hogeboom
影响因子：
2.3
作者：
G. Henehan;K. Ward;N. Kennedy;D. Weir;K. Tipton
通讯作者：
K. Tipton