H1N1 influenza virus epitopes classified by monoclonal antibodies.
H1N1 influenza virus epitopes classified by monoclonal antibodies.
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DOI：
10.3892/etm.2018.6429
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发表时间：
2018-09
影响因子：
2.7
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Guo C;Zhang H;Xie X;Liu Y;Sun L;Li H;Yu P;Hu H;Sun J;Li Y;Feng Q;Zhao X;Liang D;Wang Z;Hu J
表位在流感感染中起重要作用。分析多种亚型血凝素（HA）蛋白的表位，可能有助于筛选通用流感病毒疫苗。先前从流感病毒HA抗原获得的总共40种单克隆抗体（mAb）（先前公开了使用H1N1流感病毒裂解疫苗产生的40种mAb的开发和表征）用于使用ELISA方法检测mAb并将其分类为不同的流感病毒亚类。随后，通过与GenBank数据库和DNAMAN软件的多序列比对分析来鉴定共同的连续氨基酸序列，用于预测HA蛋白的表位。制备这些共同序列的合成肽，并用于通过定位和分布分析来验证和确定预测的线性表位。利用这些方法，鉴定了分布在不同流感病毒株中的9个HA线性表位，其中3个来自甲型流感，4个来自2009年H1N1和季节性流感，2个来自H1。本研究表明，考虑到抗原-抗体反应特异性的组合，流感病毒HA蛋白和线性表位的变异可能为设计有效的多表位疫苗提供有用的方法。本研究旨在阐明流感病毒HA诱导流感的病因和致病机制，并为其他病原微生物的抗原表位鉴定提供新的思路。
Epitopes serve an important role in influenza infection. It may be useful to screen universal influenza virus vaccines, analyzing the epitopes of multiple subtypes of the hemagglutinin (HA) protein. A total of 40 monoclonal antibodies (mAbs) previously obtained from flu virus HA antigens (development and characterization of 40 mAbs generated using H1N1 influenza virus split vaccines were previously published) were used to detect and classify mAbs into distinct flu virus sub-categories using the ELISA method. Following this, the common continuous amino acid sequences were identified by multiple sequence alignment analysis with the GenBank database and DNAMAN software, for use in predicting the epitopes of the HA protein. Synthesized peptides of these common sequences were prepared, and used to verify and determine the predicted linear epitopes through localization and distribution analyses. With these methods, nine HA linear epitopes distributed among different strains of influenza virus were identified, which included three from influenza A, four from 2009 H1N1 and seasonal influenza, and two from H1. The present study showed that considering a combination of the antigen-antibody reaction specificity, variation in the influenza virus HA protein and linear epitopes may present a useful approach for designing effective multi-epitope vaccines. Furthermore, the study aimed to clarify the cause and pathogenic mechanism of influenza virus HA-induced flu, and presents a novel idea for identifying the epitopes of other pathogenic microorganisms.
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