Is Angiostatin Involved in Physiological Foveal Avascularity?
Is Angiostatin Involved in Physiological Foveal Avascularity?
复制标题
血管抑制素是否与生理性中心凹无血管有关？
DOI：
10.1167/iovs.16-19286
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发表时间：
2016
影响因子：
4.4
通讯作者：
Thanos S
中科院分区：
文献类型：
--
作者：
Böhm MR;Hodes F;Brockhaus K;Hummel S;Schlatt S;Melkonyan H;Thanos S
目的：灵长类动物的中央视网膜具有无血管的中央凹和轮廓分明的中央凹周围毛细血管丛。在视网膜发育的任何阶段，血管和伴随的星形胶质细胞都不会进入中央凹;血管生成和血管生成抑制因子的平衡可能会在整个生命过程中维持中央凹无血管性。本研究的目的是确定参与无血管灵长类动物视网膜中央凹发展的潜在血管排斥因素。研究了新生、幼年和成年猕猴和食蟹猴的视网膜以及对照人视网膜，以确定血管抑素相对于III β-微管蛋白、胶质细胞酸性蛋白、血管内皮生长因子（VEGF）、血小板内皮细胞粘附分子-1（PECAM）和血管抑素受体αvβ3-整联蛋白。使用免疫组织化学（IHC）对视网膜整体和石蜡切片进行表达研究，并对冷冻材料进行Western印迹。结果：血管抑素在所有视网膜中均有终生表达。不同标记物联合标记显示视网膜神经节细胞是血管抑素在灵长类视网膜中表达的主要来源，而PECAM免疫阳性毛细血管表达血管抑素受体αvβ3-整合素，毛细血管相关星形胶质细胞表达VEGF。血管抑素在无血管中心凹区域和周边视网膜中的表达提示血管抑素可能在视网膜血管形成的调节中起作用，为无血管中央凹的发展和持续提供了一个可能的解释。
Purpose: The primate central retina is characterized by an avascular fovea and well-defined perifoveal capillary plexus. Neither blood vessels nor their accompanying astrocytes enter the fovea during any stage of retinal development; a balance of angiogenic and angiostatic factors probably maintains foveal avascularity throughout life. The aim of this study was to identify potentially angiorepulsive factors involved in the development of the avascular primate retinal fovea.Methods: Retinas of newborn, juvenile, and adult Callithrix jacchus and Macaca fascicularis monkeys and control human retinas were studied to determine the localization of angiostatin relative to III β-tubulin, glial fibrillary acidic protein, vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (PECAM), and the angiostatin receptor αvβ3-integrin in the foveal, macular, and peripheral retina. Expression studies were performed using immunohistochemistry (IHC) on retinal whole-mount and paraffin sections, and Western blotting on frozen material. The complex network of the main retinal cell types was identified by IHC of retinal whole mounts.Results: In general, lifetime expression of angiostatin was found in all retinas. Colabeling with different markers revealed retinal ganglion cells as the main source of angiostatin expression in the primate retina, whereas PECAM-immunopositive blood capillaries expressed the angiostatin receptor αvβ3-integrin, and capillary-associated astrocytes expressed VEGF.Conclusions: This study provides the first evidence of angiostatin expression in the primate retina; the expression of angiostatin in the avascular foveal region and the peripheral retina suggests that angiostatin may play a role in the regulation of retinal vascularization, providing a possible explanation for the development and persistence of an avascular fovea.
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DOI：
--
发表时间：
1974
期刊：
Proceedings of the Royal Society of London. Series B. Biological Sciences
影响因子：
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作者：
S. Fisher;B. Boycott
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影响因子：
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DOI：
--
发表时间：
1996
期刊：
Investigative ophthalmology & visual science.
影响因子：
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作者：
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通讯作者：
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影响因子：
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通讯作者：
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