Investigation of relationships between transverse relaxation rate, diffusion coefficient, and labeled cell concentration in ischemic rat brain using MRI.
Investigation of relationships between transverse relaxation rate, diffusion coefficient, and labeled cell concentration in ischemic rat brain using MRI.
复制标题
使用MRI研究横向松弛率，扩散系数和标记的细胞浓度之间的关系。
DOI：
10.1002/mrm.21480
复制
发表时间：
2009-03
影响因子：
3.3
通讯作者：
Chopp, Michael
中科院分区：
文献类型：
--
作者：
Athiraman, Hernanthkumar;Jiang, Quan;Ding, Guang Liang;Zhang, Li;Zhang, Zheng Gang;Wang, Lei;Arbab, Ali S.;Li, Qingjiang;Panda, Swayam;Ledbetter, Karen;Rad, Ali M.;Chopp, Michael
MRI已用于评价标记细胞的迁移和分布。然而，使用MRI的标记细胞浓度的定量测定尚未进行系统的研究。在目前的研究中，我们调查标记细胞浓度和MRI参数的横向弛豫率，R2，表观扩散系数（ADC）之间的关系，在体外的幻影和在体内的大鼠中风后。在体外，铁浓度或标记细胞浓度与R2、ADC和ADC×R2的MRI测量值之间检测到显著相关性。相反，体内标记细胞浓度与R2、ADC和ADC×R2无显著相关性。标记细胞浓度和体内MRI测量值之间不存在显著相关性的主要因素可能归因于缺血组织的背景效应。通过校正缺血性损伤引起的背景效应，ΔR2（有和无标记细胞的缺血组织中R2值的差异）显示出与标记细胞浓度的显著相关性。我们的研究表明，MRI参数有可能在体内定量测定标记的细胞浓度。
MRI has been used to evaluate labeled cell migration and distribution. However, quantitative determination of labeled cell concentration using MRI has not been systematically investigated. In the current study, we investigated the relationships between labeled cell concentration and MRI parameters of transverse relaxation rate, R2, and apparent diffusion coefficient (ADC), in vitro in phantoms and in vivo in rats after stroke. Significant correlations were detected between iron concentration or labeled cell concentration and MRI measurements of R2, ADC, and ADC×R2 in vitro. In contrast, in vivo labeled cell concentration did not significantly correlate with R2, ADC, and ADC×R2. A major factor for the absence of a significant correlation between labeled cell concentration and MRI measurements in vivo may be attributed to background effects of ischemic tissue. By correcting the background effects caused by ischemic damage, ΔR2 (difference in R2 values in the ischemic tissue with and without labeled cells) exhibited a significant correlation to labeled cell concentration. Our study suggests that MRI parameters have the potential to quantitatively determine labeled cell concentration in vivo.
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