Adherent platelet morphology on adsorbed fibrinogen: effects of protein incubation time and albumin addition.
Adherent platelet morphology on adsorbed fibrinogen: effects of protein incubation time and albumin addition.
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吸附的纤维蛋白原上的粘附血小板形态：蛋白质孵育时间和白蛋白添加的影响。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
I. Feuerstein
中科院分区：
文献类型：
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作者：
J. Sheppard;W. G. McClung;I. Feuerstein
吸附到聚合物上的蛋白质层的组成被认为对于血小板的粘附是重要的。粘附血小板的活化状态是一个额外的因素，可能是生物相容性的预测因子。活化是指从盘状形状到几种铺展形状中的任何一种的变化程度。与血小板膜上的受体（如纤维蛋白原、纤连蛋白和血管性血友病因子）相互作用的吸附的粘附蛋白的构象和取向对于血小板粘附和活化也可能是重要的。这项工作涉及的行为，纤维蛋白原吸附到PMMA单独，其中的实验变量是孵育时间与基板，并与吸附的纤维蛋白原与白蛋白混合，其中的实验变量是纤维蛋白原的摩尔百分比的吸附溶液。较短的蛋白质孵育时间和增加的白蛋白水平在初始纤维蛋白原吸附溶液中增强了活化血小板形态的百分比，并增加了血小板吸附的纤维蛋白原再分布。初始吸附溶液中较低浓度的白蛋白增强血小板粘附数;纤维蛋白原孵育时间没有影响。总之，这些因素可以通过其对血小板粘附和活化的影响而有助于生物材料的生物相容性。
The composition of the protein layer adsorbed to a polymer has been thought to be important for the adhesion of platelets. The state of activation of adherent platelets is an additional factor that may be a predictor of biocompatibility. Activation refers to the degree of change from discoid shape to any of several spread shapes. The conformation and orientation of adsorbed adhesive proteins, which interact with receptors on the membrane of platelets, such as fibrinogen, fibronectin, and von Willebrand factor, may also be important for platelet adhesion and activation. This work deals with the behavior of fibrinogen adsorbed to PMMA alone, where the experimental variable was incubation time with the substrate, and with adsorbed fibrinogen mixed with albumin, where the experimental variable was the molar percent of fibrinogen in the adsorption solution. Shorter protein incubation times and increased albumin levels in the initial fibrinogen adsorption solution enhanced the percentages of activated platelet morphologies and increased adsorbed fibrinogen redistribution by the platelet. Lower concentrations of albumin in the initial adsorption solution enhanced platelet adhesion numbers; fibrinogen incubation time had no effect. Together, these factors can contribute to the biocompatibility of a biomaterial through their effect on platelet adhesion and activation.
DOI：
10.1002/jbm.820250311
发表时间：
1991-03
期刊：
Journal of biomedical materials research
影响因子：
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作者：
K. Park;F. Mao;H. Park
通讯作者：
K. Park;F. Mao;H. Park