Integration of Metabolomics and Transcriptomicsto Comprehensively Evaluate the Metabolic Effects of Gelsemium elegans on Pigs.
Integration of Metabolomics and Transcriptomicsto Comprehensively Evaluate the Metabolic Effects of Gelsemium elegans on Pigs.
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DOI：
10.3390/ani11051192
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发表时间：
2021-04-21
期刊：
影响因子：
--
通讯作者：
Liu ZY
中科院分区：
文献类型：
--
作者：
Huang CY;Yang K;Cao JJ;Wang ZY;Wu Y;Sun ZL;Liu ZY
一些天然植物源性饲料添加剂含有多种活性化合物，已被证明是传统抗生素的有效替代品。钩吻（Gelsemium elegans（G. elegans）作为传统中草药使用多年，对猪等动物有明显的促生长作用。据我们所知，G.线虫在动物身上的作用还不清楚。在此，对G.本文首次报道了线虫在猪体内的感染情况，并研究了饲喂G. elegans 45天。结果表明，添加2%G.线虫粉饲料对猪无毒。此外，G.秀丽隐杆线虫可作为植物源性饲料添加剂，提高仔猪免疫功能，并对秀丽隐杆线虫的潜在作用机制进行了探讨。elegans可能与多种信号通路有关，包括MAPK信号通路和PPAR信号通路。总之，本研究的结果为G.本研究对该植物源性饲料添加剂的安全合理应用具有重要意义。一些含有多种活性化合物的天然植物源性饲料添加剂已被证明是传统抗生素的有效替代品。钩吻（Gelsemium elegans（G. elegans）是马钱科中的全草。它是一种已知的有毒植物，广泛分布于中国，多年来一直被用作传统的中草药，用于治疗神经性疼痛、类风湿性疼痛、炎症、皮肤溃疡和癌症。然而，G.线虫不仅对猪、羊等动物无毒，而且具有明显的促生长作用。据我们所知，G.线虫在动物身上的作用还不清楚。本研究的目的是评价饲喂仔猪G.基于转录组学和代谢组学的组合，将elegans培养45天。根据生长测定和评价结果，与全饲粮相比，添加20 g/kg G。线虫粉显著降低了仔猪基础日粮的饲料转化率。肝脏转录组的结果表明，甘氨酸和半胱氨酸相关的调节途径，包括MAPK信号通路和mTOR信号通路，在G. elegans诱导的仔猪。血浆代谢组学在正离子和负离子模式下分别鉴定出21种和18种差异代谢物（p < 0.05），G。elegans暴露和完全饮食组。甘氨酸及其衍生物和N-乙酰半胱氨酸在G.实验结果表明，线虫暴露组与对照组相比，差异有显著性。elegans可作为抗生素的替代物，提高仔猪的免疫功能，并对elegans的潜在作用机制进行了探讨。elegans可能与多种信号通路有关，包括MAPK信号通路和PPAR信号通路。
Some natural phytogenic feed additives, which contain several active compounds, have been shown to be effective alternatives to traditional antibiotics. Gelsemium elegans (G. elegans) has been used as a traditional Chinese herbal medicine for many years, and it has an obvious growth-promoting effect on animals such as pigs. To our knowledge, the internal mechanism of the influence of G. elegans on the animal body is still unclear. Here, the plasma metabolomics and liver transcriptional profile of crude extract of G. elegans in pigs were reported for the first time and the metabolic consequences of feeding piglets G. elegans for 45 days were evaluated. The results showed that the addition of 2% G. elegans powder to feed is nontoxic to pigs. In addition, G. elegans could be used as a phytogenic feed additive, which could improve the immune function of piglets, and the latent mechanism of G. elegans may be related to various signaling pathways, including the MAPK signaling pathway and PPAR signaling pathway. Collectively, results of the current provide a clearer understanding of the molecular mechanism of the pharmacological effects of G. elegans, which is of great significance for a safer and more rational application of this phytogenic feed additives. Some naturalphytogenic feed additives, which contain several active compounds, have been shown to be effective alternatives to traditional antibiotics. Gelsemium elegans (G. elegans) is a whole grass in the family Loganiaceae. It is a known toxic plant widely distributed in China and has been used as a traditional Chinese herbal medicine for many years to treat neuropathic pain, rheumatoid pain, inflammation, skin ulcers, and cancer. However, G. elegans not only is nontoxic to animals such as pigs and sheep but also has an obvious growth-promoting effect. To our knowledge, the internal mechanism of the influence of G. elegans on the animal body is still unclear. The goal of this work is to evaluate the metabolic consequences of feeding piglets G. elegans for 45 days based on the combination of transcriptomics and metabolomics. According to growth measurement and evaluation, compared with piglets fed a complete diet, adding 20 g/kg G. elegans powder to the basal diet of piglets significantly reduced the feed conversion ratio. Results of the liver transcriptome suggest that glycine and cysteine-related regulatory pathways, including the MAPK signaling pathway and the mTOR signaling pathway, were extensively altered in G. elegans-induced piglets. Plasma metabolomics identified 21 and 18 differential metabolites (p < 0.05) in the plasma of piglets in the positive and negative ion modes, respectively, between G. elegans exposure and complete diet groups. The concentrations of glycine and its derivatives and N-acetylcysteine were higher in the G. elegans exposure group than in the complete diet group.This study demonstrated that G. elegans could be an alternative to antibiotics that improves the immune function of piglets, and the latent mechanism of G. elegans may be related to various signaling pathways, including the MAPK signaling pathway and the PPAR signaling pathway.
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