Qingyi granules ameliorate severe acute pancreatitis in rats by modulating the gut microbiota and serum metabolic aberrations.
Qingyi granules ameliorate severe acute pancreatitis in rats by modulating the gut microbiota and serum metabolic aberrations.
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DOI：
10.1080/13880209.2023.2222755
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发表时间：
2023-12
影响因子：
3.8
通讯作者：
Shang, Dong
中科院分区：
文献类型：
--
作者：
Jiao, Juying;Liu, Jianjun;Luo, Fei;Shang, Mengxue;Pan, Chen;Qi, Bing;Zhao, Liang;Yin, Peiyuan;Shang, Dong
清胰颗粒可有效治疗重症急性胰腺炎（SAP）。探讨肠道菌群介导的代谢在清胰颗粒疗效中的作用。SD大鼠随机分为假手术组、SAP模型组、清胰颗粒干预组（Q，1.8g/kg）和大黄素干预组（E，50 mg/kg），观察24 h。采用H&E染色和ELISA进行组织病理学分析和血清酶及细胞因子测定。16 S rDNA测序和UHPLC-HRMS用于肠道微生物群分析和非靶向代谢组学。清胰颗粒能降低SAP大鼠胰腺病理评分（Q，7.4 ± 1.14; SAP，11.6 ± 1.14，p < 0.01）;血清淀粉酶（Q，121.2 ± 6.7; SAP，144.3 ± 8.86，p < 0.05），脂肪酶（Q，566 ± 20.34; SAP，656.7 ± 29.32，p < 0.01），（Q，492.8 ± 26.08; SAP，566.1 ± 26.83，p < 0.05）活性;（Q，29.48 ± 0.88; SAP，36.17 ± 1.88，p < 0.01）、IL-6（Q，112.2 ± 3.57; SAP，128.9 ± 9.09，p < 0.05）和TNF-α（Q，215.3 ± 8.67; SAP，266.4 ± 28.03，p < 0.05）水平。SAP诱导螺杆菌和乳酸菌过度生长，抑制罗布氏菌和异芽胞菌生长，并引起细菌代谢产物的畸变，清胰颗粒可部分逆转上述作用。清胰颗粒可通过调节肠道菌群和代谢异常来改善SAP。多组学方法允许系统地研究危重病复方的药理机制。
Qingyi granules can be used to effectively treat patients with severe acute pancreatitis (SAP). To elucidate the role of gut microbiota-mediated metabolism in the therapeutic effects of Qingyi granules. Sprague–Dawley rats were grouped into the sham operation, SAP model, Qingyi granule intervention (Q, 1.8 g/kg) and emodin intervention (E, 50 mg/kg) groups and observed for 24 h. H&E staining and ELISA were used for histopathological analysis and serum enzyme and cytokine assays. 16S rDNA sequencing and UHPLC-HRMS were used for gut microbiota analysis and untargeted metabolomics. In SAP rats, Qingyi granules decreased the pancreatic pathological score (Q, 7.4 ± 1.14; SAP, 11.6 ± 1.14, p < 0.01); serum amylase (Q, 121.2 ± 6.7; SAP, 144.3 ± 8.86, p < 0.05), lipase (Q, 566 ± 20.34; SAP, 656.7 ± 29.32, p < 0.01), and diamineoxidase (Q, 492.8 ± 26.08; SAP, 566.1 ± 26.83, p < 0.05) activities; and IL-1β (Q, 29.48 ± 0.88; SAP, 36.17 ± 1.88, p < 0.01), IL-6 (Q, 112.2 ± 3.57; SAP, 128.9 ± 9.09, p < 0.05) and TNF-α (Q, 215.3 ± 8.67; SAP, 266.4 ± 28.03, p < 0.05) levels. SAP induced Helicobacter and Lactobacillus overgrowth and suppressed Romboutsia and Allobaculum growth and caused aberrations in bacterial metabolites, which were partly reversed by Qingyi granules. Qingyi granules can modulate the gut microbiota and metabolic abnormalities to ameliorate SAP. Multi-omics approaches allow systematic study of the pharmacological mechanisms of compound prescriptions for critical illnesses.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1152/ajprenal.00271.2014
发表时间：
2014-08-15
影响因子：
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作者：
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