Ontogeny of immunoreactive calcitonin gene‐related peptide in thyroid C cells from dogs, rabbits, and guinea pigs
Ontogeny of immunoreactive calcitonin gene‐related peptide in thyroid C cells from dogs, rabbits, and guinea pigs
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狗、兔和豚鼠甲状腺 C 细胞中免疫反应性降钙素基因相关肽的个体发育
DOI：
10.1002/ar.1092200110
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Y. Kameda
中科院分区：
文献类型：
--
作者：
Y. Kameda
通过免疫过氧化物酶法研究了犬、兔和豚鼠从早期胎儿到成年甲状腺C细胞中免疫反应性降钙素基因相关肽（CGRP）的个体发育，并与免疫反应性降钙素和生长抑素的发育进行比较。CGRP在成熟C细胞中的免疫反应性的存在是不同的物种。狗和兔C细胞显示强烈的CGRP免疫反应，而豚鼠C细胞显示非常弱的免疫反应或没有。在犬胎中，CGRP免疫反应性的出现较早。妊娠35天左右，当滤泡细胞尚未组织成滤泡时，C细胞中开始出现三种肽--降钙素、生长激素抑制素和CGRP的免疫反应性。当原始卵泡在整个甲状腺实质中蓬勃形成时，生长抑素阳性细胞的数量达到最高，此后其频率逐渐下降，CGRP阳性细胞以及降钙素阳性细胞的数量和强度随着胎龄的增加而逐渐增加。狗C细胞CGRP免疫反应阳性的发育模式与降钙素免疫反应阳性的发育模式一致。在兔胎儿，在妊娠25天，当甲状腺滤泡储存大量的胶体和C细胞已经表现出强烈的降钙素，CGRP免疫反应以及生长抑素免疫反应开始出现。随后，三种肽的免疫反应性随着年龄的增长而逐渐增加，尽管降钙素免疫反应性在其中非常强烈。在豚鼠C细胞中，在任何阶段或发育中均未观察到CGRP的强烈免疫反应性。这些结果表明，每只动物都有CGRP特征的发育概况，并且C细胞中降钙素基因产生的CGRP和降钙素的比例似乎终生固定。
Ontogeny of immunoreactive calcitonin gene‐related peptide (CGRP) in thyroid C cells of dogs, rabbits, and guinea pigs from early fetuses to adults was investigated by an immunoperoxidase method, in comparison with the development of immunoreactive calcitonin and somatostatin. The presence of immunoreactive CGRP in mature C cells was different from species to species. Dog and rabbit C cells revealed intense immunoreactivity for CGRP, whereas guinea pig C cells revealed very weak immunoreactivity or none. In dog fetuses, the appearance of immunoreactive CGRP was early. At around 35 days of gestation, when the follicular cells were not yet organized into follicles, immunoreactivities for three peptides—calcitonin, somatostatin, and CGRP—began to appear in C cells. While the highest population of somatostatin‐positive cells was attained when the primordial follicles were vigorously formed throughout whole thyroid parenchyma and their frequency progressively declined thereafter, CGRP‐positive cells as well as calcitonin‐positive cells gradually increased in number and intensity with gestational age. The developmental pattern of immunoreactive CGRP coincided with that of immunoreactive calcitonin in dog C cells. In rabbit fetuses, at 25 days of gestation, when thyroid follicles stored large amounts of colloid and C cells already exhibited intense immunoreactivity for calcitonin, CGRP immunoreactivity as well as somatostatin immunoreactivity began to appear. Subsequently, immunoreactivities for the three peptides gradually increased with age, although calcitonin immunoreactivity was outstandingly intense among them. In guinea pig C cells, intense immunoreactivity for CGRP was not observed in any stages or development. These results indicate that there are developmental profiles of CGRP characteristic for each animal, and the ratio of CGRP and calcitonin produced from calcitonin genes in C cells seems to be fixed for life.
通过降钙素基因表达产生的非降钙素肽的体内和体外钙调节作用的评估。
影响因子：
4.8
作者：
Roos,BA;Fischer,JA;Pignat,W;Alander,CB;Raisz,LG
通讯作者：
Raisz,LG
脑内注射降钙素基因相关肽抑制大鼠胃酸分泌。
影响因子：
6.1
作者：
Taché,Y;Gunion,M;Lauffenberger,M;Goto,Y
通讯作者：
Goto,Y
影响因子：
4.8
作者：
DSOUZA, SM;MACINTYRE, I;MUNDY, GR
通讯作者：
MUNDY, GR