Handling of single DNA molecule using electric field and laser beam
Handling of single DNA molecule using electric field and laser beam
复制标题
使用电场和激光束处理单个 DNA 分子
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
S. Katsura
中科院分区：
文献类型：
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作者：
A. Mizuno;M. Nishioka;Tomoki Tanizoe;S. Katsura
准确处理单个 DNA 分子将支持长 DNA 分子测序的新技术。光静电显微操作（OEMM）方法已被应用于处理单个 DNA 分子。由于布朗运动，DNA 分子经常改变其位置和形状，因此精确处理很困难。琼脂糖凝胶对样品的胶凝可有效抑制布朗运动。演示了琼脂糖凝胶中单个DNA分子的基本处理技术：（1）拉伸形状固定； (2) 紫外激光切割，(3) 隔离并回收到毛细管中（内径 15 /spl mu/m）。本研究的下一步是通过 PCR（聚合酶链式反应）方法扩增回收的 DNA 片段。显微操作可能有助于加快 DNA 测序速度。<<ETX>>
Accurate handling of a single DNA molecule will support a novel technique for sequencing of a long DNA molecule. The opto-electrostatic micromanipulation (OEMM) method has been applied to handle single DNA molecules. The DNA molecule is frequently changing its position and shape due to Brownian motion, therefore precise handling is difficult. Gelation of the sample by agarose gel is effective to suppress Brownian motion. Fundamental handling techniques for the single DNA molecule in agarose gel were demonstrated: (1) fixation with stretched shape; (2) cutting by ultraviolet laser, (3) isolation and recovery into the capillary (i.d. 15 /spl mu/m). Amplification of the recovered DNA fragment by the PCR (polymerase chain reaction) method is the next step of this study. The micromanipulation may contribute to the speeding up of the DNA sequencing.<<ETX>>
影响因子：
56.9
作者：
SCHWARTZ, DC;LI, XJ;WANG, YK
通讯作者：
WANG, YK
影响因子：
56.9
作者：
SMITH, SB;FINZI, L;BUSTAMANTE, C
通讯作者：
BUSTAMANTE, C