Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation.
Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation.
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DOI：
10.1038/nsmb.3015
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发表时间：
2015-06
影响因子：
16.8
通讯作者：
Hurley, James H.
中科院分区：
文献类型：
--
作者：
Yang, Bei;Stjepanovic, Goran;Shen, Qingtao;Martin, Andreas;Hurley, James H.
AAA+ ATP酶Vps 4分解ESCRT-III，对HIV-1出芽和其他途径至关重要。Vps 4是一类六聚体AAA+ ATP酶的典型成员，其分解蛋白质复合物而不降解。为了区分复杂拆解的局部置换与全局解折叠机制，我们在嵌合Vps 24 -2 ESCRT-III细丝的酿酒酵母Vps 4拆解过程中进行了氢-氘交换。EX 1交换行为显示Vps 4在与分解反应一致的时间尺度上完全解折叠ESCRT-III底物。已建立的解折叠酶ClpX显示出相同的模式，证明了共同的解折叠机制。在孔环中含有单个半胱氨酸残基的Vps 4六聚体与在折叠的核心结构域中含有独特半胱氨酸的ESCRT-III亚基交联。这些数据支持一种机制，其中Vps 4通过完全展开它们并将它们穿过中心孔来分解其底物。
The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation. To distinguish between local displacement versus global unfolding mechanisms for complex disassembly, we carried out hydrogen-deuterium exchange during Saccharomyces cerevisiae Vps4 disassembly of of a chimeric Vps24-2 ESCRT-III filament. EX1 exchange behavior shows that Vps4 completely unfolds ESCRT-III substrates on a time scale consistent with the disassembly reaction. The established unfoldase ClpX showed the same pattern, demonstrating a common unfolding mechanism. Vps4 hexamers containing a single cysteine residue in the pore loops were cross-linked to ESCRT-III subunits containing unique cysteine within the folded core domain. These data support a mechanism in which Vps4 disassembles its substrates by completely unfolding them and threading them through the central pore.
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DOI：
10.1126/science.1161070
发表时间：
2008-09-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lata S;Schoehn G;Jain A;Pires R;Piehler J;Gottlinger HG;Weissenhorn W
通讯作者：
Weissenhorn W
DOI：
10.1016/j.bbamcr.2011.06.007
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Baker TA;Sauer RT
通讯作者：
Sauer RT
影响因子：
7.7
作者：
Cashikar, Anil G.;Shim, Soomin;Hanson, Phyllis I.
通讯作者：
Hanson, Phyllis I.
影响因子：
10.5
作者：
Gottesman, S;Roche, E;Sauer, RT
通讯作者：
Sauer, RT
影响因子：
56.9
作者：
Guizetti, Julien;Schermelleh, Lothar;Gerlich, Daniel W.
通讯作者：
Gerlich, Daniel W.