Sublethal concentrations of Au (III), Pd (II), and Ni(II) differentially alter inflammatory cytokine secretion from activated monocytes.
Sublethal concentrations of Au (III), Pd (II), and Ni(II) differentially alter inflammatory cytokine secretion from activated monocytes.
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Au (III)、Pd (II) 和 Ni(II) 的亚致死浓度会差异性地改变活化单核细胞的炎症细胞因子分泌。
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Bouillaguet
中科院分区：
文献类型：
--
作者：
J. Wataha;J. Lewis;K. R. Volkmann;P. Lockwood;R. Messer;S. Bouillaguet
许多过渡金属被认为是细胞内的非特异性生物毒素，这限制了对它们可能的治疗作用的研究。在目前的研究中，Au（III），Ni（II）和Pd（II）对单核细胞细胞因子的差异分泌的影响进行了研究。这对于了解这些金属的治疗潜力、它们的过敏性或当前金属疗法（如金疗法）的临床效果至关重要。金属的致死浓度（定义为线粒体琥珀酸脱氢酶（SDH）活性抑制> 50%）通过使用暴露于人THP-1单核细胞72小时的剂量-反应曲线来确定。然后，在单核细胞暴露于亚致死浓度的金属后，在有或没有脂多糖刺激的情况下，测量TNF α、IL 1 β和IL 6的分泌。发现抑制SDH活性50%所需的Au（III）、Pd（II）和Ni（II）的浓度分别为255、270和90 μ M。没有亚致死浓度的任何金属单独引起的细胞因子的分泌。然而，LPS诱导的细胞因子分泌显着和差异改变亚致死浓度的每种金属。差异反应高度依赖于金属浓度，并涉及抑制和增强的LPS激活。在Ni（II）的情况下，TNF α、IL 1 β和IL 6的增强范围从TNF α的200%到IL 6的1200%以上。金属如Au（III）、Pd（II）和Ni（II）差异性地改变单核细胞的细胞因子表达。这些结果意味着金属对细胞信号传导的影响比以前假设的更具体。这些结果在解释金疗法常见的多种临床效应、确定金属治疗的潜在新途径以及了解镍等金属的炎症效应方面也很重要。
Many transition metals have been viewed collectively as nonspecific biological toxins in cells, which has limited investigation into their possible therapeutic effects. In the current study, the effects of Au(III), Ni(II), and Pd(II) on the differential secretion of cytokines from monocytes has been investigated. This is critical to understanding any therapeutic potential of these metals, their allergenicity, or the clinical effects of current metal therapies such as chrysotherapy. Lethal concentrations (defined as > 50% suppression of mitochondrial succinate dehydrogenase (SDH) activity) of metals were determined by dose-response curves with the use of 72 h exposures to human THP-1 monocytes. Then, secretion of TNFalpha, IL1beta, and IL6 were measured after the monocytes were exposed to sublethal concentrations of metals, with or without stimulation by lipopolysaccharide. The concentrations of Au(III), Pd(II), and Ni(II) required to suppress SDH activity by 50% were found to be 255, 270, and 90 microM, respectively. No sublethal concentration of any metal alone caused secretion of the cytokines. However, LPS-induced cytokine secretion was significantly and differentially altered by sublethal concentrations of each metal. Differential responses were highly dependent on metal concentration and involved both suppression and potentiation of the LPS activation. In the case of Ni(II), potentiation of TNFalpha, IL1beta, and IL6 ranged from 200% for TNFalpha to over 1200% for IL6. Metals such as Au(III), Pd(II), and Ni(II) differentially alter cytokine expression from monocytes. These results imply that metals have more specific effects on cell signaling than previously assumed. These results also are important in explaining multiple clinical effects often seen with chrysotherapy, identifying potential new avenues for metal therapy, and understanding the inflammatory effects of metals such as nickel.
影响因子：
3.5
作者：
PAGE, RC
通讯作者：
PAGE, RC
影响因子：
14
作者：
ANDERSON, JM;MILLER, KM
通讯作者：
MILLER, KM
金属阳离子对真核细胞代谢的体外影响。
DOI：
10.1002/jbm.820250907
发表时间：
1991
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Wataha,JC;Hanks,CT;Craig,RG
通讯作者：
Craig,RG