Biosynthesis of prostaglandins by isolated and cultured airway epithelial cells.
Biosynthesis of prostaglandins by isolated and cultured airway epithelial cells.
复制标题
通过分离和培养的气道上皮细胞生物合成前列腺素。
DOI：
10.3109/01902148609057506
复制
发表时间：
1986
影响因子：
1.7
通讯作者：
T. Eling
中科院分区：
文献类型：
--
作者：
G. L. Xu;K. Sivarajah;R. Wu;P. Nettesheim;T. Eling
采用高效液相色谱法检测新鲜分离培养的呼吸道上皮细胞对花生四烯酸代谢前列腺素和凝血素的影响。新鲜分离的大鼠和兔气管上皮细胞制备的匀浆不能将花生四烯酸转化为前列腺素。然而，大鼠气管上皮细胞确实将花生四烯酸转化为未表征的代谢物，可能是羟基脂肪酸。相比之下，培养9天的大鼠气管上皮细胞获得了将花生四烯酸转化为PGE2及相关产物的能力，而培养的家兔气管上皮细胞则将花生四烯酸转化为TXB2。研究了大鼠气管细胞在不同培养时期花生四烯酸向PGE2转化的情况。PGE2的形成似乎与培养物的生长平行。与新鲜分离的大鼠气管细胞相比，富集的大鼠Clara细胞组分能够将14c -花生四烯酸转化为前列腺环素（PGI2），并通过HPLC分析其稳定的终产物6-酮to PGF1 α来测定。PGI2也是富集的大鼠肺泡II型细胞部分产生的花生四烯酸的主要代谢物。PGF2 α和羟基脂肪酸也形成了。这些结果表明，花生四烯酸的代谢在不同类型的呼吸道细胞中是不同的，在培养中维持这些细胞会改变花生四烯酸的代谢模式。
The metabolism of arachidonic acid to prostaglandins and thromboxane by freshly isolated and cultured respiratory tract epithelial cells was examined by HPLC methods. Homogenates prepared from freshly isolated rat and rabbit tracheal epithelial cells did not convert arachidonic acid to prostaglandins. Rat tracheal epithelial cells however, did convert arachidonic acid to uncharacterized metabolites possibly hydroxyfatty acids. In contrast, rat tracheal epithelial cells grown in culture for 9 days acquired the capacity to convert arachidonic acid to PGE2 and related products while cultured rabbit tracheal epithelial cells converted arachidonic acid to TXB2. The conversion of arachidonic acid to PGE2 by rat tracheal cells was studied at various times in culture. The formation of PGE2 appeared to parallel the growth of the cultures. In contrast to freshly isolated rat tracheal cells, enriched rat Clara cell fractions were able to convert 14C-arachidonic acid to prostacyclin (PGI2) ans measured by HPLC analysis of its stable end product 6-keto PGF1 alpha. PGI2 was also the major metabolite of arachidonic acid produced by enriched rat alveolar type II cell fractions. PGF2 alpha, and hydroxyfatty acids were also formed. These results suggest that arachidonic acid metabolism differs in various types of respiratory tract cells and that maintenance of such cells in culture alters the pattern of arachidonic acid metabolism.
犬气管上皮细胞的花生四烯酸代谢。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eling,TE;Danilowicz,RM;Henke,DC;Sivarajah,K;Yankaskas,JR;Boucher,RC
通讯作者：
Boucher,RC
狗气管上皮细胞选择性产生白三烯 B4。
DOI：
10.1016/0006-291x(83)90671-x
发表时间：
1983
影响因子：
3.1
作者：
Holtzman,MJ;Aizawa,H;Nadel,JA;Goetzl,EJ
通讯作者：
Goetzl,EJ