Activatable organic near-infrared fluorescent probes based on a bacteriochlorin platform: synthesis and multicolor in vivo imaging with a single excitation.
Activatable organic near-infrared fluorescent probes based on a bacteriochlorin platform: synthesis and multicolor in vivo imaging with a single excitation.
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DOI：
10.1021/bc4005238
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发表时间：
2014-02-19
影响因子：
4.7
通讯作者：
Kobayashi, Hisataka
中科院分区：
文献类型：
--
作者：
Harada, Toshiko;Sano, Kohei;Sato, Kazuhide;Watanabe, Rira;Yu, Zhanqian;Hanaoka, Hirofumi;Nakajima, Takahito;Choyke, Peter L.;Ptaszek, Marcin;Kobayashi, Hisataka
近红外(NIR)荧光探针是活体成像的理想选择，因为它们提供了更深的组织穿透性和更低的本底自发荧光。虽然这一范围内的大多数荧光团都是菁类染料，但已经开发了几种新的荧光近红外探测器。在这项研究中，我们开发了有机细菌素衍生物NMP4和NMP5，它们在单一的绿光激发下，在近红外光谱中发出不同的窄而清晰的谱带(NMP4和NMP5的峰值分别为739和770 nm)。当这些试剂与半乳糖-人血清白蛋白或葡萄糖-人血清白蛋白结合时，它们都针对H型凝集素，包括表达在卵巢癌上的β-d-半乳糖受体，这些试剂成为靶向、可激活、单激发、多色的近红外荧光探针。在与Glu-HSA或hGSA结合后，荧光发生显著的猝灭，这种猝灭在细胞结合和内化后被逆转。体外研究表明，与相同的Glu-HSA偶联物相比，与hGSA偶联的NMP4或NMP5对癌细胞的摄取更高。在体内，将这些药物注射到播散性卵巢癌小鼠体内后，进行单激发双色成像。在单一绿光的激发下，检测到每个荧光团的不同的近红外发射光谱，并可以通过光谱分解来区分。体内实验结果显示，用红色荧光蛋白(RFP)标记的播散性卵巢癌肿瘤模型对所有探针都有很高的敏感性和特异性。单次激发双色近红外荧光成像的成功，为进一步探索基于非菁染料的近红外荧光团奠定了基础。
Near infrared (NIR) fluorescent probes are ideal for in vivo imaging because they offer deeper tissue penetration and lower background autofluorescence. Although most fluorophores in this range are cyanine-based dyes, several new classes of fluorescent NIR probes have been developed. In this study, we developed organic bacteriochlorin derivatives, NMP4 and NMP5, which are excited with a single green light and emit different narrow, well-resolved bands in the NIR (peak of 739 and 770 nm for NMP4 and NMP5, respectively). When conjugated to galactosyl-human serum albumin (hGSA) or glucosyl-human serum albumin (glu-HSA), both targeting H-type lectins, including the β-d-galactose receptor expressing on ovarian cancer, these agents become targeted, activatable, single excitation, multicolor NIR fluorescence probes. After conjugation to either glu-HSA or hGSA, substantial quenching of fluorescence occurs that is reversed after cell binding and internalization. In vitro studies showed higher cancer cell uptake with NMP4 or NMP5 conjugated to hGSA compared to the same conjugates with glu-HSA. In vivo single excitation two-color imaging was performed after intraperitoneal injection of these agents into mice with disseminated ovarian cancer. Excited with a single green light, distinct NIR emission spectra from each fluorophore were detected and could be distinguished with spectral unmixing. In vivo results using a red fluorescence protein (RFP) labeled tumor model of disseminated ovarian cancer demonstrated high sensitivity and specificity for all probes. The success of single excitation, 2-color NIR fluorescence imaging with a new class of bacteriochlorin-based activatable fluorophores, NMP4 and NMP5, paves the way for further exploration of noncyanine dye-based NIR fluorophores.
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影响因子：
3.1
作者：
Keereweer, Stijn;Kerrebijn, Jeroen D. F.;van Driel, Pieter B. A. A.;Xie, Bangwen;Kaijzel, Eric L.;Snoeks, Thomas J. A.;Que, Ivo;Hutteman, Merlijn;van der Vorst, Joost R.;Mieog, J. Sven D.;Vahrmeijer, Alexander L.;van de Velde, Cornelis J. H.;de Jong, Robert J. Baatenburg;Lowik, Clemens W. G. M.
通讯作者：
Lowik, Clemens W. G. M.
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru
DOI：
10.1016/j.bbrc.2006.07.169
发表时间：
2006-09-29
影响因子：
3.1
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
通讯作者：
Kobayashi, Hisataka
基于物理和生物学的下一代分子成像探针的合理化学设计：混合方式，颜色和信号。
影响因子：
46.2
作者：
Kobayashi H;Longmire MR;Ogawa M;Choyke PL
通讯作者：
Choyke PL
影响因子：
3.3
作者：
Lindsey, Jonathan S.;Mass, Olga;Chen, Chih-Yuan
通讯作者：
Chen, Chih-Yuan