A Search for Ligninolytic Peroxidases in the FungusPleurotus eryngii Involving α-Keto-γ-Thiomethylbutyric Acid and Lignin Model Dimers
A Search for Ligninolytic Peroxidases in the FungusPleurotus eryngii Involving α-Keto-γ-Thiomethylbutyric Acid and Lignin Model Dimers
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杏鲍菇真菌中涉及α-酮-γ-硫代甲基丁酸和木质素模型二聚体的木质素分解过氧化物酶的研究
DOI：
10.1128/aem.65.3.916-922.1999
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发表时间：
1999
影响因子：
4.4
通讯作者：
Angel T. Martı́nez
中科院分区：
文献类型：
--
作者：
L. Caramelo;M. Martínez;Angel T. Martı́nez
由于对侧耳属植物产生的木质素降解酶存在一些争议，因此，用α-酮-γ-硫代甲基丁酸（KTBA）释放的乙烯来评估侧耳属植物培养物和细胞外蛋白的氧化能力，正如先前对黄孢原毛平革菌木质素过氧化物酶（LiP）的描述。木质素模型二聚体用于确认从液体和固态发酵（SSF）培养物中分离的酶的木质素分解能力。鉴定了在藜芦醇和H2 O2存在下氧化KTBA的三种蛋白质（在液体培养物中发现两种蛋白质，在SSF培养物中发现一种蛋白质）。这些蛋白质是多功能的过氧化物酶，作用于Mn 2+，以及简单的酚和藜芦醇。从液体培养物中获得的两种过氧化物酶能够降解非酚类β-O-4二聚体，产生藜芦醛，以及不能被黄孢原毛平革菌过氧化物酶有效氧化的酚类二聚体。前一个反应是LiP的特征反应。第三KTBA-氧化过氧化物酶氧化的酚二聚体（在Mn 2+的存在下）。最后，在SSF培养物中鉴定了第四种Mn 2+氧化过氧化物酶，其基于其在Mn 2+存在下氧化KTBA的能力。该酶与P. chrysosporium的Mn依赖性过氧化物酶相关，因为它不表现出与藜芦醇的活性和与二聚体的Mn非依赖性活性。这些结果表明，P. erynalgum产生三种类型的过氧化物酶，其具有氧化木质素的能力，但缺乏典型的LiP。类似的酶（就N-末端序列和催化性质而言）由其他的Plepherus物种产生。一些结构方面的P. eryntophan过氧化物酶的催化性能进行了讨论。
ABSTRACT Because there is some controversy concerning the ligninolytic enzymes produced by Pleurotus species, ethylene release from α-keto-γ-thiomethylbutyric acid (KTBA), as described previously for Phanerochaete chrysosporium lignin peroxidase (LiP), was used to assess the oxidative power ofPleurotus eryngii cultures and extracellular proteins. Lignin model dimers were used to confirm the ligninolytic capabilities of enzymes isolated from liquid and solid-state fermentation (SSF) cultures. Three proteins that oxidized KTBA in the presence of veratryl alcohol and H2O2 were identified (two proteins were found in liquid cultures, and one protein was found in SSF cultures). These proteins are versatile peroxidases that act on Mn2+, as well as on simple phenols and veratryl alcohol. The two peroxidases obtained from the liquid culture were able to degrade a nonphenolic β-O-4 dimer, yielding veratraldehyde, as well as a phenolic dimer which is not efficiently oxidized by P. chrysosporium peroxidases. The former reaction is characteristic of LiP. The third KTBA-oxidizing peroxidase oxidized only the phenolic dimer (in the presence of Mn2+). Finally, a fourth Mn2+-oxidizing peroxidase was identified in the SSF cultures on the basis of its ability to oxidize KTBA in the presence of Mn2+. This enzyme is related to the Mn-dependent peroxidase of P. chrysosporium because it did not exhibit activity with veratryl alcohol and Mn-independent activity with dimers. These results show that P. eryngii produces three types of peroxidases that have the ability to oxidize lignin but lacks a typical LiP. Similar enzymes (in terms of N-terminal sequence and catalytic properties) are produced by other Pleurotus species. Some structural aspects of P. eryngii peroxidases related to the catalytic properties are discussed.
DOI：
10.1073/pnas.90.4.1242
发表时间：
1993-02-15
影响因子：
11.1
作者：
KUAN, IC;TIEN, M
通讯作者：
TIEN, M
Phanerochaete chrysosporium 木质素酶对木质素模型二聚体进行氧化 C α-C β 裂解的机制。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hammel,KE;Tien,M;Kalyanaraman,B;Kirk,TK
通讯作者：
Kirk,TK
木质素过氧化物酶也可以氧化锰。
影响因子：
2.9
作者：
Khindaria,A;Barr,DP;Aust,SD
通讯作者：
Aust,SD