Multiple H4 histone mRNAs of HeLa cells are encoded in different genes
Multiple H4 histone mRNAs of HeLa cells are encoded in different genes
复制标题
HeLa细胞的多个H4组蛋白mRNA由不同基因编码
作者：
A. Lichtler;F. Sierra;S. Clark;J. Wells;J. Stein;G. Stein
我们以前曾报道存在至少两个不同的mRNA编码组蛋白H4在HeLaS 3细胞1，2。在这篇文章中，我们报告了至少七种不同的组蛋白H4 mRNA的分辨率，每一种都编码组蛋白H4。编码相同蛋白质的不同mRNA的存在可以通过不同基因的存在来解释，这些基因在不改变编码的氨基酸序列的情况下已经分化（在人类细胞中每个单倍体基因组中有20-40个组蛋白基因拷贝3），或者通过相同初级转录物的差异加工来解释。通过将一系列克隆的人类基因组H4组蛋白序列与组蛋白H4 mRNA杂交并用S1核酸酶消化，我们已经表明不同的H4组蛋白mRNA种类被不同的H4组蛋白基因保护免于核酸酶消化，这表明至少三种HeLa H4组蛋白mRNA是不同基因的产物。
We have previously reported the presence of at least two distinct mRNAs coding for histone H4 in HeLaS3 cells1,2. In this communication we report the resolution of at least seven different histone H4 mRNAs, each of which codes for histone H4. The presence of different mRNAs coding for the same protein could be explained either by the presence of different genes that have diverged without changing the encoded amino acid sequence (there are 20–40 copies of the histone genes per haploid genome in human cells3), or by differential processing of the same primary transcript. By hybridizing a series of cloned genomic human H4 histone sequences to the histone H4 mRNAs and digesting with S1 nuclease, we have shown that different H4 histone mRNA species are protected from nuclease digestion by different H4 histone genes, suggesting that at least three of the HeLa H4 histone mRNAs are the products of distinct genes.
组蛋白基因在鸡基因组中聚集但不串联重复。
DOI：
10.1073/pnas.78.5.2856
发表时间：
1981
影响因子：
11.1
作者：
Engel,JD;Dodgson,JB
通讯作者：
Dodgson,JB