Long-Term Live-Cell STED Nanoscopy of Primary and Cultured Cells with the Plasma Membrane HIDE Probe DiI-SiR.
Long-Term Live-Cell STED Nanoscopy of Primary and Cultured Cells with the Plasma Membrane HIDE Probe DiI-SiR.
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与质膜隐藏探针DII-SIR的原代和培养细胞的长期活细胞纳米镜检查。
DOI：
10.1002/anie.201704783
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发表时间：
2017-08-21
期刊：
影响因子：
--
通讯作者：
Schepartz A
中科院分区：
文献类型：
--
作者：
Thompson AD;Omar MH;Rivera-Molina F;Xi Z;Koleske AJ;Toomre DK;Schepartz A
Super-resolution imaging of live cells over extended time periods with high temporal resolution requires high-density labeling and extraordinary fluorophore photostability. Here we achieve this goal by combining the attributes of the high-density plasma membrane probe DiI-TCO and the photostable STED dye SiR-Tz. These components undergo rapid tetrazine ligation within the plasma membrane to generate the HIDE probe DiI-SiR. Using DiI-SiR, we visualized filopodia dynamics in HeLa cells over 25 min at 0.5 sec temporal resolution, and visualized dynamic contact-mediated repulsion events in primary mouse hippocampal neurons over 9 min at 2 sec temporal resolution. HIDE probes such as DiI-SiR are non-toxic and do not require transfection, and their apparent photostability significantly improves the ability to monitor dynamic processes in live cells at super-resolution over biologically relevant timescales.
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树突稳定性的分子机制。
影响因子：
34.7
作者：
Koleske, Anthony J.
通讯作者：
Koleske, Anthony J.
DOI：
10.1002/anie.201403349
发表时间：
2014-09-15
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Erdmann RS;Takakura H;Thompson AD;Rivera-Molina F;Allgeyer ES;Bewersdorf J;Toomre D;Schepartz A
通讯作者：
Schepartz A
影响因子：
15
作者：
Grunwald, Christian;Schulze, Katrin;Tampe, Robert
通讯作者：
Tampe, Robert
影响因子：
5.3
作者：
Lauterbach, Marcel Andreas;Guillon, Marc;Emiliani, Valentina
通讯作者：
Emiliani, Valentina
影响因子：
21.8
作者：
Lukinavicius, Grazvydas;Umezawa, Keitaro;Johnsson, Kai
通讯作者：
Johnsson, Kai