Ultrastructural changes of the basilar artery following experimental subarachnoid haemorrhage
Ultrastructural changes of the basilar artery following experimental subarachnoid haemorrhage
复制标题
实验性蛛网膜下腔出血后基底动脉超微结构的变化
DOI：
10.1007/bf01403606
复制
发表时间：
1989
影响因子：
2.4
通讯作者：
E. Reale
中科院分区：
文献类型：
--
作者：
V. Seifert;D. Stolke;E. Reale
最近的实验研究表明，蛛网膜下腔出血后，脑血管内皮发生了显著的变化，可能导致内皮表面的生化变化，血管舒张和血管收缩机制的微妙稳态受到干扰，这被认为是维持脑血管张力的原因。结合不同形式的SAH后内皮的显微观察的超微结构研究是进一步研究脑血管痉挛发病机制的先决条件，但这些研究很少。本实验研究是为了更准确地研究和定义实验性SAH后犬基底动脉内皮表面的病理形态学变化。在72小时内向大池分别注射两次自体血液导致所有动物基底动脉直径广泛的血管造影变窄。基底动脉的组织学研究，包括光镜、透射电镜、扫描电镜和冷冻开裂显微镜检查，显示内皮表面有严重的病理形态学改变。主要表现为内皮的内折叠和皱折，内皮细胞的失位和脱屑，以及内皮层和肌层之间纤维组织的空泡化和向内生长。肌层未见病理形态学改变。由于痉挛时脑血管内皮出血后超微结构的改变可能代表了脑血管痉挛迟发型的形态学基础，未来对其发病机制的研究应主要关注SAH后脑血管内皮表面发生的结构和生化变化。
SummaryRecent experimental studies have shown, that the endothelium of cerebral vessels undergoes significant changes after subarachnoid haemorrhage which may lead to biochemical changes at the endothelial surface with disturbance of the delicate homeostasis of vasodilating and vasoconstricting mechanisms which are thought to be responsible for preservation of the tones of the cerebral vasculature. Ultrastructural studies incorporating different forms of microscopic observations of the endothelium after SAH representing a prerequisite for further investigations on the pathogenesis of cerebral vasospasm are scarce. This experimental study was performed in order to investigate and define more precisely the pathomorphological changes at the endothelial surface of the basilar artery of dogs after experimental SAH.Two separate injections of autologous blood into the cisterna magna within 72 hours resulted in extensive angiographic narrowing of the diameter of the basilar artery of all animals. Histological studies of the basilar artery including light microscopic, transmission electron microscopic, scanning electron microscopic and freeze cracking microscopic examinations demonstrated severe pathomorphological changes at the endothelial surface. These consisted mainly of infolding and corrugation of the endothelium, disorientation and desquamation of endothelial cells as well as of vacuolation and ingrowth of fibrous tissue between the endothelial and muscular layer. No pathomorphological changes could be observed in the muscular layer.As the described post-haemorrhagic ultrastructural changes of the endothelium of cerebral vessels in spasm are likely to represent the morphological basis of the delayed form of cerebral vasospasm future research on its pathogenesis should primarily focus on the structural and biochemical changes taking place at the endothelial surface of the cerebral vasculature after SAH.
影响因子：
4.1
作者：
T. Liszczak;P. Black;A. Tzouras;L. Foley;N. Zervas
通讯作者：
T. Liszczak;P. Black;A. Tzouras;L. Foley;N. Zervas
DOI：
--
发表时间：
1983
期刊：
The Physiologist
影响因子：
--
作者：
Kontos,HA;Wei,EP;Christman,CW;Levasseur,JE;Povlishock,JT;Ellis,EF
通讯作者：
Ellis,EF