Dose-Response Study of Microcystin Congeners MCLA, MCLR, MCLY, MCRR, and MCYR Administered Orally to Mice.
Dose-Response Study of Microcystin Congeners MCLA, MCLR, MCLY, MCRR, and MCYR Administered Orally to Mice.
复制标题
小鼠口服微囊藻毒素同源物MCLA、MCLY、MCRR和MCYR的剂量反应研究。
DOI：
10.3390/toxins13020086
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发表时间：
2021-01-24
期刊：
影响因子：
4.2
通讯作者：
Huang H
中科院分区：
文献类型：
--
作者：
Chernoff N;Hill D;Lang J;Schmid J;Farthing A;Huang H
微囊藻毒素是一种常见的淡水蓝藻毒素，影响肝功能。在BALB/c小鼠中，对有害藻华中常见的5种微囊藻毒素同系物(微囊藻毒素-LA、MCLR、MCLY、MCRR和MCYR)进行了毒性评价，剂量范围从未观察到的不良反应水平(NOAELs)到最低观察不良反应水平(LOAELs)。监测动物的行为和外表的变化，并在给药后24小时安乐死。试验终点包括临床变化，尸检观察，以及肝脏毒性和总体动态平衡的血清指标。剂量分别为0.5~7 mg/kg MCLA、0.5~11 mg/kg MCLR、1~7 mg/kg MCLY、7~22 mg/kg MCRR、3~11 mg/kg MCYR。MCLA 3 mg/kg可升高肝/体重比和肝脏评分、ALT、AST和GLDH，表明肝毒性，降低血糖，显著升高血清总胆红素。MCLR和MCLY与LOAELs 5 mg/kg的作用相似，但在MCLR动物中观察到的影响更大和更严重。MCRR暴露在22 mg/kg时与血糖降低有关。MCYR在7 mg/kg剂量下可引起散发性肝损伤，在5 mg/kg剂量下可降低血糖水平。结果表明，微囊藻毒素暴露后，同系物诱导的毒性有显著差异。
Microcystins are common freshwater cyanobacterial toxins that affect liver function. The toxicities of five microcystin congeners (microcystin-LA (MCLA), MCLR, MCLY, MCRR, and MCYR) commonly observed in harmful algal blooms (HABs) were evaluated in BALB/c mice after a single oral administration of doses ranging from those that were no observed adverse effect levels (NOAELs) to lowest observed adverse effect levels (LOAELs). Animals were monitored for changes in behavior and appearance, and euthanized 24 h after dosing. Test endpoints included clinical changes, necropsy observations, and serum indicators of hepatic toxicity and general homeostasis. Doses were 0.5–7 mg/kg MCLA, 0.5–11 mg/kg MCLR, 1–7 mg/kg MCLY, 7–22 mg/kg MCRR, and 3–11 mg/kg MCYR. MCLA at 3 mg/kg elevated liver/body weight ratio and liver score, ALT, AST, and GLDH, indicating hepatic toxicity, reduced serum glucose and highly elevated total serum bilirubin. MCLR and MCLY induced similar effects with LOAELs of 5 mg/kg, although a greater extent and severity of effects were observed in MCLR animals. MCRR exposure at 22 mg/kg was associated with reduced serum glucose. MCYR induced scattered liver effects at 7 mg/kg and reduced serum glucose levels at 5 mg/kg. The results indicate significant differences in congener-induced toxicity after microcystin exposure.
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影响因子：
4.2
作者：
Foss, Amanda J.;Aubel, Mark T.;Fogelson, Susan B.
通讯作者：
Fogelson, Susan B.
影响因子：
2.8
作者：
Fawell, JK;Mitchell, RE;Hill, RE
通讯作者：
Hill, RE
影响因子：
2.8
作者：
HARADA, K;MATSUURA, K;CARMICHAEL, WW
通讯作者：
CARMICHAEL, WW
影响因子：
3.7
作者：
Dolman AM;Rücker J;Pick FR;Fastner J;Rohrlack T;Mischke U;Wiedner C
通讯作者：
Wiedner C
影响因子：
--
作者：
Dreher, Theo W;Collart, Lindsay P;Ferry, Rodney
通讯作者：
Ferry, Rodney