Deoxynivalenol Impairs Weight Gain and Affects Markers of Gut Health after Low-Dose, Short-Term Exposure of Growing Pigs.
Deoxynivalenol Impairs Weight Gain and Affects Markers of Gut Health after Low-Dose, Short-Term Exposure of Growing Pigs.
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DOI：
10.3390/toxins7062071
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发表时间：
2015-06-09
期刊：
影响因子：
4.2
通讯作者：
Fink-Gremmels J
中科院分区：
文献类型：
--
作者：
Alizadeh A;Braber S;Akbari P;Garssen J;Fink-Gremmels J
脱氧雪腐镰刀菌烯醇(DON)是镰刀菌产生的主要真菌毒素之一，暴露在这种真菌毒素下需要评估其潜在的不良影响，即使在低毒素水平下也是如此。这项研究的目的是调查短期、低剂量DON暴露对猪各种肠道健康参数的影响。仔猪饲喂商品饲料或DON(0.9 mg/kg饲料)污染饲料10天，注射DON(0.28 mg/kg体重)后2小时测定增重并采集不同肠段的样品。即使在饮食中选择低剂量的DON也会对体重增加产生负面影响，并导致十二指肠和空肠的组织形态改变。不同紧密连接(TJ)蛋白，尤其是封闭蛋白，炎症标志物，如白介素1β和白介素10，以及氧化应激标志物血红素氧合酶1，在肠道中的表达受到低水平DON的影响。综上所述，我们的结果表明，即使在动物饲料中被普遍认为可以接受的低水平接触DON之后，与临床相关的变化在肠道健康和完整性的标记物中也是可以测量的。
Deoxynivalenol (DON) is one of the major mycotoxins produced by Fusarium fungi, and exposure to this mycotoxin requires an assessment of the potential adverse effects, even at low toxin levels. The aim of this study was to investigate the effects of a short-term, low-dose DON exposure on various gut health parameters in pigs. Piglets received a commercial feed or the same feed contaminated with DON (0.9 mg/kg feed) for 10 days, and two hours after a DON bolus (0.28 mg/kg BW), weight gain was determined and samples of different segments of the intestine were collected. Even the selected low dose of DON in the diet negatively affected weight gain and induced histomorphological alterations in the duodenum and jejunum. The mRNA expression of different tight junction (TJ) proteins, especially occludin, of inflammatory markers, like interleukin-1 beta and interleukin-10 and the oxidative stress marker heme-oxigenase1, were affected along the intestine by low levels of DON in the diet. Taken together, our results indicate that even after low-level exposure to DON, which has been generally considered as acceptable in animal feeds, clinically-relevant changes are measurable in markers of gut health and integrity.
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影响因子：
3.8
作者：
Amuzie, Chidozie J.;Pestka, James J.
通讯作者：
Pestka, James J.
肠道猪上皮细胞的基因调节IPEC-J2取决于脱氧基因维烯醇毒理学作用部位。
影响因子：
3.7
作者：
Diesing AK;Nossol C;Ponsuksili S;Wimmers K;Kluess J;Walk N;Post A;Rothkötter HJ;Kahlert S
通讯作者：
Kahlert S
影响因子：
2.8
作者：
Dinu, Diana;Bodea, Gabriela Oana;Dinischiotu, Anca
通讯作者：
Dinischiotu, Anca
影响因子：
4.1
作者：
Basuroy, S;Seth, A;Rao, R
通讯作者：
Rao, R
影响因子：
3
作者：
Daenicke, Sven;Valenta, Hana;Rothkoetter, Hermann-Josef
通讯作者：
Rothkoetter, Hermann-Josef