Investigation of Telomerase/Telomeres system in Bone Marrow Mesenchymal Stem Cells derived from IPF and RA-UIP.
Investigation of Telomerase/Telomeres system in Bone Marrow Mesenchymal Stem Cells derived from IPF and RA-UIP.
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DOI：
10.1186/1476-9255-9-27
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发表时间：
2012-07-02
期刊：
影响因子：
--
通讯作者：
Siafakas NM
中科院分区：
文献类型：
--
作者：
Antoniou KM;Margaritopoulos GA;Proklou A;Karagiannis K;Lasithiotaki I;Soufla G;Kastrinaki MC;Spandidos DA;Papadaki HA;Siafakas NM
特发性肺纤维化和风湿性关节炎相关的普通型间质性肺炎似乎有同样的不良结局，因为没有有效的治疗方法。本研究的目的是通过对端粒酶/端粒系统的评价来探讨骨髓间充质干细胞的修复能力，并提出一种新的治疗方法。在6名IPF患者、7名RA-UIP患者和6名健康对照中研究BM-MSC。我们评估了端粒长度以及端粒酶的两种组分（人端粒酶逆转录酶，h-TERT和与端粒缺失DNA互补的RNA模板，h-TERC）的mRNA表达。我们发现，来自IPF、RA-UIP病例的BM-MSC不呈现比对照更小的端粒长度（p = 0.170）。患者和健康对照之间的h-TERT和h-TERC基因的表达没有显著差异（分别为p = 0.107和p = 0.634）。我们证明IPF和RA-UIP的BM-MSCs中端粒长度和端粒酶表达相同，这可以解释发病机制和预后的相似性。这些细胞中端粒长度的维持可能在这些破坏性肺部疾病的干细胞替代治疗中具有未来意义。
Idiopathic Pulmonary Fibrosis and Rheumatoid Arthritis associated usual interstitial pneumonia seem to have the same poor outcome as there is not an effective treatment. The aim of the study is to explore the reparative ability of bone marrow mesenchymal stem cells by evaluating the system telomerase/telomeres and propose a novel therapeutic approach. BM-MSCs were studied in 6 IPF patients, 7 patients with RA-UIP and 6 healthy controls. We evaluated the telomere length as well as the mRNA expression of both components of telomerase (human telomerase reverse transcriptase, h-TERT and RNA template complementary to the telomeric loss DNA, h-TERC). We found that BM-MSCs from IPF, RA-UIP cases do not present smaller telomere length than the controls (p = 0.170). There was no significant difference regarding the expression of both h-TERT and h-TERC genes between patients and healthy controls (p = 0.107 and p = 0.634 respectively). We demonstrated same telomere length and telomerase expression in BM-MSCs of both IPF and RA-UIP which could explain similarities in pathogenesis and prognosis. Maintenance of telomere length in these cells could have future implication in cell replacement treatment with stem cells of these devastating lung disorders.
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影响因子：
24
作者：
Hare, Joshua M.;Traverse, Jay H.;Henry, Timothy D.;Dib, Nabil;Strumpf, Robert K.;Schulman, Steven P.;Gerstenblith, Gary;DeMaria, Anthony N.;Denktas, Ali E.;Gammon, Roger S.;Hermiller, James B., Jr.;Reisman, Mark A.;Schaer, Gary L.;Sherman, Warren
通讯作者：
Sherman, Warren
DOI：
10.1164/rccm.200804-550oc
发表时间：
2008-10-01
影响因子：
24.7
作者：
Cronkhite, Jennifer T.;Xing, Chao;Garcia, Christine Kim
通讯作者：
Garcia, Christine Kim
影响因子：
4
作者：
Izadpanah, Reza;Trygg, Cynthia;Bunnell, Bruce A.
通讯作者：
Bunnell, Bruce A.
影响因子：
20.3
作者：
Beyth, S;Borovsky, Z;Rachmilewitz, J
通讯作者：
Rachmilewitz, J
影响因子：
4.3
作者：
Antoniou, Katerina M.;Papadaki, Helen A.;Siafakas, Nikolaos M.
通讯作者：
Siafakas, Nikolaos M.