Magic angle spinning and oriented sample solid-state NMR structural restraints combine for influenza a M2 protein functional insights.
Magic angle spinning and oriented sample solid-state NMR structural restraints combine for influenza a M2 protein functional insights.
复制标题
DOI：
10.1021/ja3004039
复制
发表时间：
2012-06-06
影响因子：
15
通讯作者：
Cross, Timothy A.
中科院分区：
文献类型：
--
作者：
Can, Thach V.;Sharma, Mukesh;Hung, Ivan;Gor'kov, Peter L.;Brey, William W.;Cross, Timothy A.
作为一种小型四聚体螺旋膜蛋白，M2质子通道结构对其环境高度敏感。因此，脂质双层环境的结构数据已被证明对于描述电导机制至关重要。虽然定向样品固态核磁共振在脂质双层中提供了高分辨率的主链结构，但螺旋的四级堆积和许多侧链构象受到的限制很差。此外，对四元结构稳定性的理解仍然是一个谜。在这里，来自脂质体制剂的魔角旋转固态NMR的各向同性化学位移数据和螺旋间交叉峰强烈支持跨膜螺旋束的四级结构作为二聚体结构的二聚体。数据还解释了在激活该质子通道之前，一旦 His37 四分体吸收两个电荷，四聚体稳定性如何增强。这两种固态核磁共振技术的结合似乎是表征螺旋膜蛋白结构的有效方法。
As a small tetrameric helical membrane protein, the M2 proton channel structure is highly sensitive to its environment. As a result, structural data from a lipid bilayer environment has proven to be essential for describing the conductance mechanism. While oriented sample solid state NMR has provided a high resolution backbone structure in lipid bilayers, quaternary packing of the helices and many of the sidechain conformations have been poorly restrained. Furthermore, an understanding of the quaternary structural stability has remained a mystery. Here, the isotropic chemical shift data and interhelical cross peaks from magic angle spinning solid state NMR of a liposomal preparation strongly support the quaternary structure of the transmembrane helical bundle as a dimer of dimers structure. The data also explains how the tetrameric stability is enhanced once two charges are absorbed by the His37 tetrad prior to activation of this proton channel. The combination of these two solid state NMR techniques appear to be a powerful approach for characterizing helical membrane protein structure.
登录
查看更多内容
影响因子：
4.8
作者：
Tang, YJ;Zaitseva, F;Pinto, LH
通讯作者：
Pinto, LH
来自固态NMR的流感M2质子通道中质子传导和门控的机制。
DOI：
10.1126/science.1191714
发表时间：
2010-10-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hu F;Luo W;Hong M
通讯作者：
Hong M
影响因子：
2.2
作者：
McNeill, Seth A.;Gor'kov, Peter L.;Long, Joanna R.
通讯作者：
Long, Joanna R.
影响因子：
3.4
作者：
Hu, Jun;Asbury, Tom;Cross, Timothy A.
通讯作者：
Cross, Timothy A.
DOI：
10.1073/pnas.0905726106
发表时间：
2009-07-28
影响因子：
11.1
作者：
Ma, Chunlong;Polishchuk, Alexei L.;Pinto, Lawrence H.
通讯作者：
Pinto, Lawrence H.