Replication Timing Properties within the Mouse Distal Chromosome 7 Imprinting Cluster
Replication Timing Properties within the Mouse Distal Chromosome 7 Imprinting Cluster
复制标题
小鼠远端染色体 7 印记簇内的复制计时特性
DOI：
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复制
发表时间：
2002
期刊：
影响因子：
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通讯作者：
K. Okumura
中科院分区：
文献类型：
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作者：
K. Kagotani;S. Takebayashi;A. Kohda;H. Taguchi;M. Paulsen;J. Walter;W. Reik;K. Okumura
基因组印记的特征是染色体区域内基因的等位基因特异性表达。在这里，我们表明，使用荧光原位杂交（FISH）分析，大的染色体结构域的小鼠远端7号染色体印迹簇，约1 Mb的长度之间的p57Kip2和H19基因，复制异步之间的两个等位基因在S期。在该结构域的端粒侧，我们发现了从印记p57Kip2基因的异步复制到Nap2基因的同步复制的过渡。双色荧光原位杂交结果表明，该区域的父系等位基因比母系等位基因早复制。用组蛋白去乙酰化酶抑制剂处理细胞，废除了这种等位基因特异性特征，并伴随着在结构域水平上后期复制等位基因的加速复制。甚至在ES细胞中也观察到等位基因特异性异步复制。这些结果表明，这个印记集群包括一个大的复制域，这是已经发现在早期阶段的发展。
Genomic imprinting is characterized by allele-specific expression of genes within chromosomal domains. Here we show, using fluorescence in situ hybridization (FISH) analysis, that the large chromosomal domain of the mouse distal chromosome 7 imprinting cluster, approximately 1 Mb in length between p57Kip2 and H19 genes, replicates asynchronously between the two alleles during S-phase. At the telomeric side of this domain, we found a transition from asynchronous replication at the imprinted p57Kip2 gene to synchronous replication at the Nap2 gene. Two-color FISH suggested that the paternal allele of this whole domain replicates earlier than its maternal allele. Treatment of the cells with a histone deacetylase inhibitor abolished this allele-specific feature accompanied with accelerated replication of the later-replicating allele at a domain level. Allele-specific asynchronous replication was observed even in ES cells. These results suggest that this imprinting cluster consists of a large replication domain which is already found at the early stage in development.
影响因子：
11.4
作者：
SELIG, S;OKUMURA, K;CEDAR, H
通讯作者：
CEDAR, H
DOI：
10.1006/bbrc.1998.9401
发表时间：
1998-10-20
影响因子：
3.1
作者：
Hu, JF;Oruganti, H;Hoffman, AR
通讯作者：
Hoffman, AR