Growth hormone bioactivity and immunoactivity in tall children
Growth hormone bioactivity and immunoactivity in tall children
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高个子儿童的生长激素生物活性和免疫活性
DOI：
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发表时间：
1999
影响因子：
5.4
通讯作者：
A. Barreca
中科院分区：
文献类型：
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作者：
M. Bozzola;G. Radetti;F. Buzi;G. Tonini,;A. Moretta;M. Autelli;A. Aglialoro;G. Rondini;A. Barreca
在体质较高的受试者中，用商业试剂盒测量时，观察到对刺激试验的生长激素反应有高有低。为了研究这些相互矛盾的结果的原因，我们使用不同的测定方法评估了生长激素（GH）的分泌以及GH结合蛋白和胰岛素样生长因子- 1 （IGF-I）在高个子儿童中的浓度。采用免疫荧光测定法（IFMA）和Nb2细胞生物测定法对22名年龄2.87 ~ 13.25岁的青春期前体质高者进行了两项药理学试验，以评估血清GH水平。采用RIA法测定血清IGF-I值。用FPLC凝胶过滤法评价循环低亲和力（LA）和高亲和力（HA） gh结合蛋白（ghbp）。考虑IFMA测定的最高血清GH水平，将22名高个受试者分为两组：A组16名血清GH峰值降低（5.78±0.68 ng/ml）， B组6名血清GH峰值正常（15.73±1.56 ng/ml）。A组（14.77±1.54 ng/ml）和B组（16.03±1.96 ng/ml）血清GH峰值水平与11名年龄和性别匹配的对照组（12.25±1.19 ng/ml）无显著差异。A组血清GH峰值水平的Nb2细胞生物测定/IFMA比值（0.29±0.08）显著高于B组（0.07±0.01）。16例A组患者与6例b组患者血清LA-GHBP、HA-GHBP及IGF-I浓度均无差异。同样，两组间的生长学参数也无差异。使用Nb2细胞生物测定法评估生长激素的生物活性，与IFMA测量的生长激素反应低的高个子儿童相比，生长激素反应正常的儿童相似，并且与生长学数据和血清IGF-I浓度一致。
In subjects with constitutional tall stature, both low and high GH response to stimulation tests have been observed when measured by commercial kits. To investigate the reason for these conflicting results, we evaluated growth hormone (GH) secretion using different assays as well as GH-binding protein and insulinlike growth factor-I (IGF-I) concentrations in tall children. Serum samples were collected from 22 prepubertal constitutionally tall children, aged 2.87–13.25 years, during two pharmacological tests to evaluate serum GH levels measured by both immunofluorometric assay (IFMA) and the Nb2 cell bioassay. Serum IGF-I values were evaluated by RIA. Circulating low affinity (LA) and high affinity (HA) GH-binding proteins (GHBPs) were evaluated by FPLC gel filtration. Considering the highest serum GH levels as measured by IFMA, the 22 tall subjects were divided into two groups: group A including 16 children with blunted serum GH peak levels (5.78±0.68 ng/ml) and group B including 6 subjects with normal serum GH peak values (15.73±1.56 ng/ml). No differences were observed in serum GH peak levels as measured by the Nb2 cell bioassay between group A (14.77±1.54 ng/ml) and group B (16.03±1.96 ng/ml), and between both groups and 11 age-and sex-matched controls (12.25±1.19 ng/ml). In group A, the Nb2 cell bioassay/IFMA ratio of serum GH peak levels (0.29±0.08) was significantly higher (p<0.05) than in group B (0.07±0.01). No differences were found in serum LA-GHBP and HA-GHBP as well as in IGF-I concentrations between the 16 patients of group A and the 6 of group B. Likewise, no difference in auxological parameters was found between the two groups. The biological activity of GH evaluated using the Nb2 cell bioassay is similar in tall children with a low GH response as measured by IFMA in comparison with those with a normal GH response, and is in agreement with both the auxological data and serum IGF-I concentrations.
影响因子：
5.8
作者：
HO, KY;EVANS, WS;THORNER, MO
通讯作者：
THORNER, MO
生长激素 (GH) 和生物体液中不同分子形式的 GH 的代谢。
影响因子：
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作者：
Baumann,G
通讯作者：
Baumann,G
体内分泌的人类生长激素的分子形式：分泌刺激的非特异性。
DOI：
10.1210/jcem-62-4-789
发表时间：
1986
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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作者：
Baumann,G;Stolar,MW
通讯作者：
Stolar,MW