Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition.
Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition.
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DOI：
10.1371/journal.pone.0102444
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Clemens S
中科院分区：
文献类型：
--
作者：
Schmidt H;Günther C;Weber M;Spörlein C;Loscher S;Böttcher C;Schobert R;Clemens S
缺铁会损害人类健康和植物生产力。因此，了解植物铁的获取策略对于作物植物的发育非常重要，这些作物在铁有限的条件下（例如碱性土壤）具有更高的铁效率，并且在其可食用组织中具有更高的铁密度。长期以来，人们一直假设根部分泌酚类化合物是非禾本科植物吸收铁的还原策略的一个组成部分。因此，我们对在富铁和贫铁条件下生长的拟南芥植物的根进行气相色谱-质谱和超高压液相色谱电喷雾电离四极杆飞行时间质谱的综合代谢组分析。在代谢物中发现了东莨菪素和其他香豆素，对两种不同的限铁条件（无铁培养基和碱性 pH 培养基中的培养）表现出最强的反应。肉桂酸邻位羟基化缺陷的香豆素生物合成突变体在没有铁肥的情况下无法在碱性土壤上生长。与野生型植物共培养部分挽救了缺铁表型，表明细胞外香豆素对铁溶解的贡献。事实上，在野生型植物的根部分泌物中检测到了香豆素。直接输注质谱以及紫外/可见光谱表明香豆素既充当 Fe(III) 的还原剂又充当 Fe(II) 的配体。
Fe deficiency compromises both human health and plant productivity. Thus, it is important to understand plant Fe acquisition strategies for the development of crop plants which are more Fe-efficient under Fe-limited conditions, such as alkaline soils, and have higher Fe density in their edible tissues. Root secretion of phenolic compounds has long been hypothesized to be a component of the reduction strategy of Fe acquisition in non-graminaceous plants. We therefore subjected roots of Arabidopsis thaliana plants grown under Fe-replete and Fe-deplete conditions to comprehensive metabolome analysis by gas chromatography-mass spectrometry and ultra-pressure liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Scopoletin and other coumarins were found among the metabolites showing the strongest response to two different Fe-limited conditions, the cultivation in Fe-free medium and in medium with an alkaline pH. A coumarin biosynthesis mutant defective in ortho-hydroxylation of cinnamic acids was unable to grow on alkaline soil in the absence of Fe fertilization. Co-cultivation with wild-type plants partially rescued the Fe deficiency phenotype indicating a contribution of extracellular coumarins to Fe solubilization. Indeed, coumarins were detected in root exudates of wild-type plants. Direct infusion mass spectrometry as well as UV/vis spectroscopy indicated that coumarins are acting both as reductants of Fe(III) and as ligands of Fe(II).
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DOI：
10.1073/pnas.0708367105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Jeong, Jeeyon;Cohu, Christopher;Guerinot, Mary Lou
通讯作者：
Guerinot, Mary Lou
影响因子：
2.1
作者：
CHANEY, RL
通讯作者：
CHANEY, RL
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
3.5
作者：
Barupal DK;Kind T;Kothari SL;Lee DY;Fiehn O
通讯作者：
Fiehn O
影响因子：
5.3
作者：
Borlotti A;Vigani G;Zocchi G
通讯作者：
Zocchi G