Diazepinone effect on liver tissue respiration and serum lipid content in rats with a rotenone model of Parkinson’s disease
Diazepinone effect on liver tissue respiration and serum lipid content in rats with a rotenone model of Parkinson’s disease
复制标题
二氮杂酮对帕金森病鱼藤酮模型大鼠肝组织呼吸和血脂含量的影响
DOI：
10.15407/ubj92.06.085
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
S. P. Vesеlsky
中科院分区：
文献类型：
--
作者：
L. Shtanova;P. Yanchuk;S. P. Vesеlsky
帕金森病（PD）是一种与年龄相关的慢性进行性神经退行性疾病。 α-突触核蛋白聚集体的积累、氧化应激、线粒体功能障碍和脂质代谢紊乱被认为是 PD 发病机制的关键损伤。尽管经过长期研究，PD的病因尚未明确。我们研究了苯二氮卓类新衍生物二氮杂卓对 28 天内腹腔注射 2.0 mg/kg 鱼藤酮 (ROT) 诱导的 PD 模型大鼠的肝组织呼吸 (lTR)、血脂含量和行为参数的影响。使用极谱仪lP-9 评估lTR。从下腔静脉采集用于生化分析的血样。通过旷场试验研究大鼠的行为参数。我们发现，在ROT诱导的PD大鼠中，肝脏耗氧系数降低了33.5%（P < 0.001），血清中磷脂、胆固醇、胆固醇酯、游离脂肪酸和甘油三酯含量降低了21.4%（P < 0.001）、28.8%（P < 0.001）、26.8%（P < 0.001）、分别有 30.3% (P < 0.01) 和 41.5% (P < 0.001) 检测到运动障碍。二氮杂酮的应用导致lTR、血清磷脂浓度的完全恢复、血清游离脂肪酸和甘油三酯含量的部分恢复以及运动行为的显着改善。然而，二氮杂酮并不影响模拟PD大鼠血清中胆固醇和胆固醇酯浓度的降低。
Parkinson’s disease (PD) is a chronic and progressive age-related neurodegenerative disorder. accumulation of α-synuclein aggregates, oxidative stress, mitochondrial dysfunction and lipid metabolism disturbance are thought to be the key impairments underlying PD pathogenesis. Despite long-time research, the causes of PD have not yet been clear. We investigated the influence of diazepinon, a new derivative of benzodiazepine, on liver tissue respiration (lTR), serum lipid content and behavioral parameters of rats with modeled PD induced by intraperitoneal injections of 2.0 mg/kg rotenone (ROT) within 28 days. lTR was assessed using the polarograph lP-9. Blood samples for biochemical analysis were collected from the inferior vena cava. The behavioral parameters of rats were studied by the open field test. We showed that in rats with ROT – induced PD, the coefficient of liver oxygen consumption was decreased by 33.5% (P < 0.001), the serum content of phospholipids, cholesterol, cholesterol esters, free fatty acids and triglycerides was reduced by 21.4% (P < 0.001), 28.8% (P < 0.001), 26.8% (P < 0.001), 30.3% (P < 0.01) and 41.5% (P < 0.001), respectively and the motor disorders were detected. Diazepinone application resulted in a full restoration of lTR, serum concentration of phospholipids, partial recovery of serum free fatty acids and triglycerides content and significant improvement of motor behavior. However, diazepinone did not affect the reduced concentration of cholesterol and cholesterol esters in the serum of rats with simulated PD.
影响因子：
4.4
作者：
Graham SF;Rey NL;Yilmaz A;Kumar P;Madaj Z;Maddens M;Bahado-Singh RO;Becker K;Schulz E;Meyerdirk LK;Steiner JA;Ma J;Brundin P
通讯作者：
Brundin P
DOI：
10.1152/ajpendo.1998.275.2.e249
发表时间：
1998-08-01
影响因子：
5.1
作者：
Gallagher, D;Belmonte, D;Heymsfield, SB
通讯作者：
Heymsfield, SB
影响因子：
5
作者：
Chen, H;Zhang, SMM;Ascherio, A
通讯作者：
Ascherio, A