Flu@home: the Comparative Accuracy of an At-Home Influenza Rapid Diagnostic Test Using a Prepositioned Test Kit, Mobile App, Mail-in Reference Sample, and Symptom-Based Testing Trigger.
Flu@home: the Comparative Accuracy of an At-Home Influenza Rapid Diagnostic Test Using a Prepositioned Test Kit, Mobile App, Mail-in Reference Sample, and Symptom-Based Testing Trigger.
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DOI：
10.1128/jcm.02070-21
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发表时间：
2022-03-16
影响因子：
9.4
通讯作者：
Thompson M
中科院分区：
文献类型：
--
作者：
Kotnik JH;Cooper S;Smedinghoff S;Gade P;Scherer K;Maier M;Juusola J;Ramirez E;Naraghi-Arani P;Lyon V;Lutz B;Thompson M
使用呼吸道病毒快速诊断测试 (RDT) 进行家庭测试可以促进早期诊断、指导患者护理并预防传播。此类 RDT 最好在疾病发作时使用，此时病毒载量最高，临床行动最有影响力，这可以通过家庭测试来实现。我们在家中评估了 QuickVue A+B 流感 RDT 的诊断准确性。在美国各地前瞻性地招募了 5,229 名参与在线健康研究平台的个人作为方便样本。在研究开始时，为参与者预先准备了包含 QuickVue RDT 和参考样本采集以及运输材料的“Flu@home”测试套件。参与者对每日症状调查做出回应。如果他们报告出现咳嗽并伴有疼痛、发烧、发冷和/或出汗，他们会按照移动应用程序上的说明使用 Flu@home 套件，并指出他们在 RDT 上看到的线条。在 976 名符合使用自我收集套件标准并完成研究程序的参与者中，202 名 (20.7%) 的 qPCR 检测结果呈流感阳性。 RDT 对于甲型流感的敏感性为 28%（95% CI = 21 至 36），特异性为 99%（98 至 99），对于乙型流感为 32%（95% CI = 20 至 46）和 99%（95% CI = 98 至 99）。不建议将其与本研究中使用的 RDT 一起使用。需要进一步的研究来确定提高家庭流感检测准确性和实用性的方法。
At-home testing with rapid diagnostic tests (RDTs) for respiratory viruses could facilitate early diagnosis, guide patient care, and prevent transmission. Such RDTs are best used near the onset of illness when viral load is highest and clinical action will be most impactful, which may be achieved by at-home testing. We evaluated the diagnostic accuracy of the QuickVue Influenza A+B RDT in an at-home setting. A convenience sample of 5,229 individuals who were engaged with an on-line health research platform were prospectively recruited throughout the United States. “Flu@home” test kits containing a QuickVue RDT and reference sample collection and shipping materials were prepositioned with participants at the beginning of the study. Participants responded to daily symptom surveys. If they reported experiencing cough along with aches, fever, chills, and/or sweats, they used their flu@home kit following instructions on a mobile app and indicated what lines they saw on the RDT. Of the 976 participants who met criteria to use their self-collection kit and completed study procedures, 202 (20.7%) were positive for influenza by qPCR. The RDT had a sensitivity of 28% (95% CI = 21 to 36) and specificity of 99% (98 to 99) for influenza A, and 32% (95% CI = 20 to 46) and 99% (95% CI = 98 to 99), for influenza B. Our results support the concept of app-supported, prepositioned at-home RDT kits using symptom-based triggers, although it cannot be recommended with the RDT used in this study. Further research is needed to determine ways to improve the accuracy and utility of home-based testing for influenza.
影响因子：
3.7
作者：
Zigman Suchsland ML;Rahmatullah I;Lutz B;Lyon V;Huang S;Kline E;Graham C;Cooper S;Su P;Smedinghoff S;Chu HY;Sewalk K;Brownstein JS;Thompson MJ;Seattle Flu Study investigators
通讯作者：
Seattle Flu Study investigators
影响因子：
3.7
作者：
Gordon A;Videa E;Saborío S;López R;Kuan G;Balmaseda A;Harris E
通讯作者：
Harris E