Enhanced level of double-stranded RNA-dependent protein kinase in peripheral blood mononuclear cells of patients with viral infections.
Enhanced level of double-stranded RNA-dependent protein kinase in peripheral blood mononuclear cells of patients with viral infections.
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病毒感染患者外周血单个核细胞中双链RNA依赖性蛋白激酶水平升高。
DOI：
10.1089/jir.1986.6.85
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
A. Hovanessian
中科院分区：
文献类型：
--
作者：
C. Buffet‐Janvresse;J. Vannier;A. Laurent;N. Robert;A. Hovanessian
在健康志愿者和不同类型病毒感染患者的外周血单核（PBM）细胞提取物中检测了依赖于双链RNA的蛋白激酶活性。在含有针对68,000 Mr蛋白（蛋白激酶的一个亚基）的单克隆抗体的免疫亲和柱上一步纯化后，检测蛋白激酶。在健康个体中，蛋白激酶的活性保持不变。相反，在病毒感染患者中，蛋白激酶的活性显著增强，在疾病过程中，随着临床症状的改善和逆转，蛋白激酶的活性降低。蛋白激酶活性水平的提高与另一种干扰素介导的酶2-5A合成酶有很强的相关性。因此，这两种酶都可以作为评估疾病状态和恢复的标记物。在不同的淋巴细胞群体中，发现大部分蛋白激酶活性存在于T淋巴细胞和B淋巴细胞中，T淋巴细胞的活性高于B淋巴细胞。
The protein kinase activity dependent on double-stranded RNA was assayed in extracts of peripheral blood mononuclear (PBM) cells from healthy volunteers and in patients with different types of virus infections. The protein kinase was assayed after one-step purification on an immunoaffinity column containing monoclonal antibody against the 68,000 Mr protein, a subunit of the protein kinase. In healthy individuals, the activity of the protein kinase remains constant. In contrast, the activity of the protein kinase is enhanced significantly in patients with viral infections and is decreased during the course of the disease in parallel with clinical ameliorations and reversal of clinical symptoms. There is a strong correlation between the enhanced levels of the protein kinase activity and another interferon-mediated enzyme, 2-5A synthetase. Both of these enzymes, therefore, could be used as markers to evaluate the state of the disease and recovery. In the different populations of lymphocytes, most of the protein kinase activity was found to be present in T and B lymphocytes, T lymphocytes showing a higher activity than B lymphocytes.
接受干扰素-α 治疗的患者中 2,5-寡腺苷酸合成酶的测量。
DOI：
10.1089/jir.1985.5.191
发表时间：
1985
期刊：
Journal of interferon research
影响因子：
--
作者：
Merritt,JA;Borden,EC;Ball,LA
通讯作者：
Ball,LA