ISOLATION OF OLIGODEOXYNUCLEOSIDE PHOSPHOROTHIOATE DIASTEREOMERS BY THE COMBINATION OF DEAE ION-EXCHANGE AND REVERSED-PHASE CHROMATOGRAPHY
ISOLATION OF OLIGODEOXYNUCLEOSIDE PHOSPHOROTHIOATE DIASTEREOMERS BY THE COMBINATION OF DEAE ION-EXCHANGE AND REVERSED-PHASE CHROMATOGRAPHY
复制标题
结合 DEAE 离子交换和反相色谱分离寡脱氧核苷硫代磷酸酯非对映体
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
K. Makino
中科院分区：
文献类型：
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作者：
H. Kanehara;M. Mizuguchi;K. Makino
摘要在反义试验中，硫代磷酸寡脱氧核苷（S-oligo）的立体异构体是由硫代磷酸的R/S构型产生的。我们已经探索了这些异构体的有效分离的DEAE离子交换（IELC）和反相（RPLC）液相色谱相结合。对26个S-寡核苷酸和一些天然对应物进行了检查。当5′-端有DMTr保护基存在时，ELC能很好地分离异构体，而当5′-端没有DMTr保护基时，RPLC能很好地分离异构体。这两种不同模式的组合导致更有效的隔离。a在水野义久博士75岁生日之际献给他。
Abstract In antisense trials, oligodeoxynucleoside phosphorothioates (S-oligo) stereoisomers arise from the thiophosphate R/S configuration. We have explored effective separation of these isomers by the combination of DEAE ion-exchange (IELC) and reversed-phase (RPLC) liquid chromatography. Twenty-six S-oligos and some of the natural counterparts were examined. In the presence of DMTr at the 5′-end, isomers could be separated well by ELC, while RPLC gave good resolution in the absence of this protecting group. Combination of these two different modes leads to more effective isolation. aDedicated to Dr. Yoshihisa Mizuno on the occasion of his 75th birthday.
寡脱氧核糖核苷酸硫代磷酸酯的离子交换高效液相色谱分析。
影响因子：
2.9
作者：
Metelev,V;Agrawal,S
通讯作者：
Agrawal,S