Irisin and exercise training in humans - results from a randomized controlled training trial.
Irisin and exercise training in humans - results from a randomized controlled training trial.
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DOI：
10.1186/1741-7015-11-235
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发表时间：
2013-11-05
期刊：
影响因子：
9.3
通讯作者：
Meyer T
中科院分区：
文献类型：
--
作者：
Hecksteden A;Wegmann M;Steffen A;Kraushaar J;Morsch A;Ruppenthal S;Kaestner L;Meyer T
最近发现的一种新的肌细胞因子（irisin）可能参与健康相关的训练效果已经获得了极大的关注，但训练诱导的irisin增加的证据仍然是初步的。因此，本研究旨在确定人类定期运动训练后鸢尾素浓度是否增加。在随机对照设计中，研究了两种符合指南的培训干预措施。入选标准为年龄30 - 60岁，每周规律活动<1小时，不吸烟，无重大疾病。102名参与者可纳入分析。训练组受试者每周运动3次，共26周。最低依从性定义为70%。有氧耐力训练（AET）包括45分钟的步行/跑步，心率储备为60%。力量耐力训练（SET）包括8个基于机器的练习（2组15次重复，最多20次重复的100%）。在训练研究结束后立即以单盲测量测定冷冻血清样品中的血清鸢尾素浓度。体能表现为训练的整体效果提供了积极的控制。使用方差分析检验组间差异的显著性。对于与对照组的事后比较，使用Dunnett检验。与对照组相比，训练组的最大运动能力显著提高（对照组：±0.0 ± 0.7 km/h; AET：1.1 ± 0.6 km/h，P < 0.01; SET：+0.5 ± 0.7 km/h，P = 0.01）。两组间鸢尾素的变化没有差异（对照组：101 ± 81 ng/ml; AET：44 ± 93 ng/ml; SET：60 ± 92 ng/ml;两种情况下：P = 0.99（单侧检验），1−β错误概率= 0.7）。总体上升趋势主要是由于鸢尾素浓度与冷冻血清样品的储存时间呈负相关（P < 0.01，β =-0.33）。在算术消除该混杂因素后，组间差异仍然不显著。无法证实训练引起的循环中鸢尾素的增加，这使人们对其参与与健康有关的训练效果提出了质疑。由于冷冻样品随着时间的推移容易发生鸢尾素降解，因此来自非对照试验的阳性结果可能完全反映了来自初始测试的样品的较长储存时间。Clinicaltrials.gov.标识符：NCT 01263522。
The recent discovery of a new myokine (irisin) potentially involved in health-related training effects has gained great attention, but evidence for a training-induced increase in irisin remains preliminary. Therefore, the present study aimed to determine whether irisin concentration is increased after regular exercise training in humans. In a randomized controlled design, two guideline conforming training interventions were studied. Inclusion criteria were age 30 to 60 years, <1 hour/week regular activity, non-smoker, and absence of major diseases. 102 participants could be included in the analysis. Subjects in the training groups exercised 3 times per week for 26 weeks. The minimum compliance was defined at 70%. Aerobic endurance training (AET) consisted of 45 minutes of walking/running at 60% heart rate reserve. Strength endurance training (SET) consisted of 8 machine-based exercises (2 sets of 15 repetitions with 100% of the 20 repetition maximum). Serum irisin concentrations in frozen serum samples were determined in a single blinded measurement immediately after the end of the training study. Physical performance provided positive control for the overall efficacy of training. Differences between groups were tested for significance using analysis of variance. For post hoc comparisons with the control group, Dunnett’s test was used. Maximum performance increased significantly in the training groups compared with controls (controls: ±0.0 ± 0.7 km/h; AET: 1.1 ± 0.6 km/h, P < 0.01; SET: +0.5 ± 0.7 km/h, P = 0.01). Changes in irisin did not differ between groups (controls: 101 ± 81 ng/ml; AET: 44 ± 93 ng/ml; SET: 60 ± 92 ng/ml; in both cases: P = 0.99 (one-tailed testing), 1−β error probability = 0.7). The general upward trend was mainly accounted for by a negative association of irisin concentration with the storage duration of frozen serum samples (P < 0.01, β = −0.33). After arithmetically eliminating this confounder, the differences between groups remained non-significant. A training-induced increase in circulating irisin could not be confirmed, calling into question its proposed involvement in health-related training effects. Because frozen samples are prone to irisin degradation over time, positive results from uncontrolled trials might exclusively reflect the longer storage of samples from initial tests. Clinicaltrials.gov. Identifier: NCT01263522.
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影响因子：
2.7
作者：
Hecksteden, Anne;Gruetters, Teresa;Meyer, Tim
通讯作者：
Meyer, Tim
影响因子：
5.1
作者：
Choi, Yeon-Kyung;Kim, Mi-Kyung;Park, Keun-Gyu
通讯作者：
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DOI：
10.1073/pnas.0903485106
发表时间：
2009-05-26
影响因子：
11.1
作者：
Ristow, Michael;Zarse, Kim;Blueher, Matthias
通讯作者：
Blueher, Matthias
DOI：
10.1152/ajpregu.00538.2010
发表时间：
2011-06-01
影响因子：
2.8
作者：
Little, Jonathan P.;Safdar, Adeel;Gibala, Martin J.
通讯作者：
Gibala, Martin J.
影响因子：
--
作者：
MIKINES, KJ;SONNE, B;GALBO, H
通讯作者：
GALBO, H