Evidence that the outer membrane protein gene nmpC of Escherichia coli K-12 lies within the defective qsr' prophage
Evidence that the outer membrane protein gene nmpC of Escherichia coli K-12 lies within the defective qsr' prophage
复制标题
大肠杆菌 K-12 的外膜蛋白基因 nmpC 位于有缺陷的 qsr 原噬菌体中的证据
DOI：
10.1128/jb.162.1.256-262.1985
复制
发表时间：
1985
影响因子：
3.2
通讯作者：
C. Schnaitman
中科院分区：
文献类型：
--
作者：
P. Highton;Y. Chang;W. Marcotte;C. Schnaitman
在nmpC（p+）突变株和nmpC+亲本中制备λ噬菌体和大肠杆菌K-12的缺陷性qsr'前噬菌体之间的融合子。来源于nmpC（p+）菌株的重组qsr'噬菌体的溶原性菌株的外膜含有电泳迁移率与NmpC孔蛋白和噬菌体PA-2编码的Lc孔蛋白相同的新蛋白。来自nmpC+菌株的qsr'重组体的溶原和携带qsr'区域的λ p4的溶原不产生该蛋白。用电子显微镜观察，从qsr'原噬菌体获得的DNA显示与含有lc基因的噬菌体PA-2的DNA分子的区域同源。相对于来自nmpC（p+）突变体的重组体，来自nmpC+亲本的重组体的DNA分子在lc基因附近含有插入。这些结果得到了E.大肠杆菌染色体与来自噬菌体PA-2的lc基因的探针。在nmpC位点发现了与lc基因同源的序列，并且亲本菌株含有插入，暂时鉴定为IS 5 B，位于孔蛋白编码序列的3'端附近。我们得出的结论是，NmpC孔蛋白的结构基因位于E. coliK-12作图，该基因可通过缺失插入元件而被激活。
Recombinants between phage lambda and the defective qsr' prophage of Escherichia coli K-12 were made in an nmpC (p+) mutant strain and in the nmpC+ parent. The outer membrane of strains lysogenic for recombinant qsr' phage derived from the nmpC (p+) strain contained a new protein identical in electrophoretic mobility to the NmpC porin and to the Lc porin encoded by phage PA-2. Lysogens of qsr' recombinants from the nmpC+ strain and lysogens of lambda p4, which carries the qsr' region, did not produce this protein. When observed by electron microscopy, the DNA acquired from the qsr' prophage showed homology with the region of the DNA molecule of phage PA-2 which contains the lc gene. Relative to that of the recombinant from the nmpC (p+) mutant, the DNA molecule of the recombinant from the nmpC+ parent contained an insertion near the lc gene. These results were supported by blot hybridization analysis of the E. coli chromosome with probes derived from the lc gene of phage PA-2. A sequence homologous to the lc gene was found at the nmpC locus, and the parental strains contained an insertion, tentatively identified as IS5B, located near the 3' end of the porin coding sequence. We conclude that the structural gene for the NmpC porin protein is located within the defective qsr' prophage at 12.5 min on the E. coli K-12 map and that this gene can be activated by loss of an insertion element.
DOI：
10.1073/pnas.81.7.1966
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MIZUNO, T;CHOU, MY;INOUYE, M
通讯作者：
INOUYE, M