In situ localization of melanotransferrin (melanoma-associated antigen P97) in human liver. A light- and electronmicroscopic immunohistochemical study.
In situ localization of melanotransferrin (melanoma-associated antigen P97) in human liver. A light- and electronmicroscopic immunohistochemical study.
复制标题
人肝脏中黑素转铁蛋白（黑色素瘤相关抗原 P97）的原位定位。
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
V. Desmet
中科院分区：
文献类型：
--
作者：
R. Sciot;R. De vos;P. Van Eyken;K. Van Der Steen;P. Moerman;V. Desmet
Using an indirect immunoperoxidase technique on frozen sections with the monoclonal antibody 96.5, we investigated the in situ distribution of melanotransferrin, a transferrin (Tf) and transferrin receptor (TfR) related glycoprotein, in human liver. Specimens included normal liver, liver in iron overload, hepatocellular carcinoma, angioma and foetal liver. On light microscopy, immunoreactivity was almost exclusively present on sinusoidal lining cells, apparently endothelial cells; the pattern was similar in normal and in iron-loaded liver. A gradient of more enhanced staining in acinar zone II and III was observed. The endothelial localization of the staining was supported by the positivity of the central vein endothelium and of the angiomas. Immunoelectron microscopy on three liver specimens showed positivity on sinusoidal endothelial cells but not on Ito and Kupffer cells. In addition, positivity on rough endoplasmic reticulum vesicles of some hepatocytes was also present. Four hepatocellular carcinomas showed an intense staining in tumour cells, 3 were weakly positive and 3 were negative. In the foetal livers, the central vein endothelium was positive from 21 weeks of gestation onward and additional positivity of zone III sinusoidal endothelial cells was present from 27 weeks on. The present results show that in the liver melanotransferrin has a localization different from Tf and the TfR. These latter molecules are predominantly localized in parenchymal cells. In addition, there does not appear to be a coordinate regulation secondary to iron storage, between melanotransferrin, Tf and the TfR. The observed gradient in the staining pattern in foetal and adult liver specimens further supports the heterogeneity of the endothelial cell population in the liver and suggests a developmental relationship between endothelial cells of sinusoids and central vein.
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p97 抗原定位于 3 号染色体的 q24-qter 区域；
影响因子：
9.8
作者：
Seligman,PA;Butler,CD;Massey,EJ;Kaur,JA;Brown,JP;Plowman,GD;Miller,Y;Jones,C
通讯作者：
Jones,C
DOI：
--
发表时间：
1987-05
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Herlyn;W. Clark;U. Rodeck;M. Mancianti;J. Jambrosic;H. Koprowski
通讯作者：
M. Herlyn;W. Clark;U. Rodeck;M. Mancianti;J. Jambrosic;H. Koprowski
肿瘤细胞不稳定、多样化和转移表型的进展：从癌基因到癌胎儿表达。
影响因子：
11.2
作者：
Nicolson,GL
通讯作者：
Nicolson,GL
色素细胞病变的抗原表型：黑色素瘤、其前体病变和转移性黑色素瘤异质性的研究。
DOI：
10.1111/1523-1747.ep12265530
发表时间：
1985
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Ernstoff,MS;Duray,P;Stenn,K;Kirkwood,JM
通讯作者：
Kirkwood,JM