Fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists
Fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists
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无铁牛乳铁蛋白对几种真菌植物病原体和拮抗剂的抑菌活性
DOI：
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发表时间：
2008
影响因子：
2.2
通讯作者：
R. Capparelli
中科院分区：
文献类型：
--
作者：
E. Lahoz;A. Pisacane;M. Iannaccone;D. Palumbo;R. Capparelli
乳铁蛋白（LF）是铁结合糖蛋白转铁蛋白家族的一员。它也是一种80 kDa的多功能蛋白，由腺上皮细胞合成并分泌到粘膜液中。高水平的LF存在于初乳和乳汁中，低水平存在于眼泪、唾液、胃肠道和生殖分泌物中。关于LF抗真菌作用的数据有限。对白色念珠菌进行的研究表明，LF抑制了这种真菌的生长。本研究报道了从植物和土壤中分离的11种真菌对LF生长影响的实验结果。这些实验采用了九种不同浓度的LF（0、0.001、0.01、0.1、1、10、100、1000、5000 mg L−1）修饰琼脂的方法。LF对这些真菌生长的影响是基于真菌菌落径向生长的测量，以抑制百分比和IC50值表示（真菌生长相对于对照抑制50%的浓度）。LF对交替稻瘟菌、玫瑰胶粘菌、枯萎菌和炭疽菌无显著影响。但对黑曲霉、绿色木霉、菌核菌、罗氏菌核菌、茄根丝核菌和绿斑病菌的生长有一定的抑制作用，其IC50值为菌核菌31.1 mg L−1和绿色丝虫病菌952 mg L−1。
Lactoferrin (LF) is a member of the transferrin family of iron-binding glycoproteins. It is also a multifunctional protein of 80 kDa that is synthesized by glandular epithelial cells and secreted into mucosal fluid. High levels of LF are present in colostrom and milk and low levels in tears, saliva, and gastrointestinal and reproductive secretions. Data regarding the antifungal effects of LF are limited. Studies have been performed on Candida albicans, which demonstrated that LF inhibits the growth of this fungus. This study reports the results of experiments carried out in order to evaluate the effects of LF on the growth of 11 fungi, which were isolated from plants and soils. These experiments employed the methods of amended agar utilizing nine different concentration levels of LF (0, 0.001, 0.01, 0.1, 1, 10, 100, 1000, 5000 mg L−1). The effects of LF on the growth of these fungi were based on measures of the radial growth of the fungal colonies expressed both as percentage of inhibition and as IC50 values (the concentration at which the fungal growth was inhibited by 50% relative to controls). LF had no effects on Alternaria alternata, Gliocladium roseum, Fusarium solani and Colletotrichum lindemuthianum. It did, however, inhibit the growth of Aspergillus niger, Trichoderma viride, Sclerotinia sclerotiorum, Sclerotium rolfsii, Rhizoctonia solani and Phoma exigua to the point that their IC50 values ranged from 31.1 mg L−1 for S. sclerotiorum to 952 mg L−1 for T. viride.
铁载体介导的 Gliocladium virens 中铁 (III) 的吸收。
影响因子：
3.9
作者：
Jalal,MA;Love,SK;vanderHelm,D
通讯作者：
vanderHelm,D
影响因子：
5.6
作者：
ANDERSON, BF;BAKER, HM;BAKER, EN
通讯作者：
BAKER, EN