Amplification of a Zygosaccharomyces bailii DNA segment in wine yeast genomes by extrachromosomal circular DNA formation.
Amplification of a Zygosaccharomyces bailii DNA segment in wine yeast genomes by extrachromosomal circular DNA formation.
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DOI：
10.1371/journal.pone.0017872
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发表时间：
2011-03-10
期刊：
影响因子：
3.7
通讯作者：
Dequin S
中科院分区：
文献类型：
--
作者：
Galeote V;Bigey F;Beyne E;Novo M;Legras JL;Casaregola S;Dequin S
我们最近描述了酿酒酵母菌株基因组中由于独立水平基因转移（HGT）事件而产生的大染色体片段的存在，这些菌株主要来自葡萄酒。我们在此报告了酿酒葡萄球菌基因组中其中一个片段的扩增证据，这是一个来自Zygosaccharomyces bailii的17 kb DNA片段。该区域的拷贝数、组织和位置在不同菌株之间差异很大，表明插入是独立的，是hgt后事件。我们在28株酿酒葡萄球菌中鉴定出8种不同的形式，其中大部分来自葡萄酒，在一个菌株中有多达4个不同的副本。这些形式的组织和在酿酒酵母中自动复制的功能序列的鉴定强烈表明，染色体外环状DNA （eccDNA）分子在酵母基因组中扩增Z. bailii区域中起着中间作用。我们在断点区域发现很少或没有序列相似性，这表明插入可能是由非同源重组介导的。酿酒葡萄球菌中这些区域之间的多样性大约代表了葡萄酒菌株基因组差异的三分之一，这证实了这一事件的最近起源，晚于葡萄酒菌株扩张的开始。这是首次报道在天然酵母群体中，通过非同源重组介导的DNA片段扩增的环状机制。
We recently described the presence of large chromosomal segments resulting from independent horizontal gene transfer (HGT) events in the genome of Saccharomyces cerevisiae strains, mostly of wine origin. We report here evidence for the amplification of one of these segments, a 17 kb DNA segment from Zygosaccharomyces bailii, in the genome of S. cerevisiae strains. The copy number, organization and location of this region differ considerably between strains, indicating that the insertions are independent and that they are post-HGT events. We identified eight different forms in 28 S. cerevisiae strains, mostly of wine origin, with up to four different copies in a single strain. The organization of these forms and the identification of an autonomously replicating sequence functional in S. cerevisiae, strongly suggest that an extrachromosomal circular DNA (eccDNA) molecule serves as an intermediate in the amplification of the Z. bailii region in yeast genomes. We found little or no sequence similarity at the breakpoint regions, suggesting that the insertions may be mediated by nonhomologous recombination. The diversity between these regions in S. cerevisiae represents roughly one third the divergence among the genomes of wine strains, which confirms the recent origin of this event, posterior to the start of wine strain expansion. This is the first report of a circle-based mechanism for the expansion of a DNA segment, mediated by nonhomologous recombination, in natural yeast populations.
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