Isolation and Characterization of Fetal Bovine Cells Derived from Liver and Bone Marrow
Isolation and Characterization of Fetal Bovine Cells Derived from Liver and Bone Marrow
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源自肝脏和骨髓的胎牛细胞的分离和表征
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
T. Takezawa
中科院分区：
文献类型：
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作者：
T. Takenouchi;T. Takezawa
为了阐明动物发育和分化、形态发生和组织再生所涉及的细胞机制，我们开发了一种从牛肝脏和骨髓中分离胎儿和成体细胞的方法。我们用编码猿猴病毒 40 大 T 抗原 (SV40LT) 和新霉素抗性基因的 DNA pSV3neo 载体转染细胞，以建立永生化细胞系，并使用免疫细胞化学和 RT-PCR 来表征细胞系。我们期望这些细胞系将有助于观察三维培养系统中各种类型的细胞行为。
In order to clarify the cellular mechanisms involved in animal development and differentiation, morphogenesis, and tissue regeneration, we have developed a method for isolating fetal as well as adult cells from bovine liver and bone marrow. We transfected the cells with a DNA pSV3neo vector that codes for simian virus 40 large T antigen (SV40LT) and neomycin-resistance genes to establish immortalized cell lines, and characterized the cell lines using by immunocytochemistry and RT-PCR. We expect that these cell lines will be useful for observing various types of cell behavior in a three-dimensional culture system.
DOI：
10.1002/1097-4636(200105)55:2
发表时间：
2001-05-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
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作者：
Martin, I;Shastri, VP;Freed, LE
通讯作者：
Freed, LE