Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis.
Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis.
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牛黄体中内皮素 1 和内皮素转化酶 1 表达的独特细胞定位和调节：对黄体溶解的影响。
DOI：
10.1210/endo.142.12.8550
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发表时间：
2001
期刊：
影响因子：
4.8
通讯作者：
R. Meidan
中科院分区：
文献类型：
--
作者：
N. Levy;M. Gordin;R. Mamluk;M. Yanagisawa;M. F. Smith;J. Hampton;R. Meidan
在自然或PGF(2α)诱导的黄体溶解过程中，黄体(CL)内的内皮素-1(ET)-1迅速上调；然而，在黄体早期，当CL对PGF(2α)无效时，这种上调没有观察到。ET-1的成熟和活性形式是由非活性中间肽BIG ET-1通过ET转换酶-1产生的。因此，本研究检测了ECE1在牛CL中的发育和细胞特异性表达。在CL从黄体早期到黄体中期的转变过程中，CEC-1的表达(mRNA和蛋白水平)显著增加了4倍。原位杂交和富集黄体细胞亚群分析表明，CL的类固醇生成细胞和内皮细胞均高水平表达ECA-1mRNA，而PreproET-1mRNA仅在常驻内皮细胞表达。这些数据表明，黄体实质细胞和内皮细胞可能在成熟的生物活性ET-1的生物合成中起协同作用。在成熟的CL中，类固醇生成细胞和内皮细胞中均有ECE1mRNA的表达增加，并伴随着ET-1多肽的显著升高。然而，在黄体早期和黄体中期CL中，ET-1前原基因的表达水平与欧洲经委会-1相反。黄体早期的低ECE1水平限制了活性ET-1的产生，这可能解释了为什么未成熟的CL能够抵抗PGF(2α)诱导的黄体溶解。
Endothelin-1 (ET)-1 within the corpus luteum (CL) is rapidly up-regulated during natural or PGF(2 alpha)-induced luteolysis; however, such an increase was not observed at early luteal stage, when the CL is refractory to PGF(2 alpha). The mature and active form of ET-1 is derived from the inactive intermediate peptide, big ET-1, by ET-converting enzyme (ECE)-1. This study therefore examined the developmental and cell-specific expression of ECE-1 in bovine CL. A significant, 4-fold, elevation in ECE-1 expression (mRNA and protein levels) occurred during the transition of the CL from early to midluteal phase. Analysis using in-situ hybridization and enriched luteal cell subpopulations showed that both steroidogenic and endothelial cells of the CL expressed high levels of ECE-1 mRNA; prepro ET-1 mRNA, on the other hand, was only expressed by resident endothelial cells. These data suggest that luteal parenchymal and endothelial cells may cooperate in the biosynthesis of mature bioactive ET-1. In the mature CL, ECE-1 mRNA increase occurred both in steroidogenic and endothelial cells and was accompanied by a significant rise in ET-1 peptide. However, in contrast to ECE-1, prepro ET-1 mRNA levels were similar in early and midluteal-phase CL. Low ECE-1 levels during the early luteal phase, restricting the production of active ET-1, may explain why the immature CL is able to withstand PGF(2 alpha)-induced luteolysis.
前列腺素 F2α 调节早期和中期牛黄体的明显生理变化。
影响因子：
3.6
作者：
Tsai,SJ;Wiltbank,MC
通讯作者：
Wiltbank,MC
影响因子：
--
作者：
Andreas Bulling;Frank Dieter Berg;U. Berg;Diane M. Duffy;R. Stouffer;S. Ojeda;Manfred Gratzl;Artur Mayerhofer
通讯作者：
Andreas Bulling;Frank Dieter Berg;U. Berg;Diane M. Duffy;R. Stouffer;S. Ojeda;Manfred Gratzl;Artur Mayerhofer