Analysis of Expressed Sequence Tags Derived from Developing Seed and Pollen Cones of Cryptomeria japonica
Analysis of Expressed Sequence Tags Derived from Developing Seed and Pollen Cones of Cryptomeria japonica
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日本柳杉种子和花粉锥发育过程中表达序列标签的分析
DOI：
10.1055/s-2003-44690
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发表时间：
2003
期刊：
影响因子：
3.9
通讯作者：
Y. Tsumura
中科院分区：
文献类型：
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作者：
T. Ujino;Yuriko Taguchi;K. Yoshimura;Y. Tsumura
我们从两个cDNA文库中获得了1210个表达序列标签（expressed sequence tags，ESTs），代表了1173个转录本。在这1173个转录本中，807个与已知序列显示出显著的相似性，包括在其他植物物种的发育花组织中表达的基因，如CONSTANS和MADS结构域蛋白的基因。这一结果表明，尽管在形态上的差异，生殖器官的发育是由一个家庭的同源转录因子共同裸子植物和被子植物物种。其余366个转录本与公共数据库中的任何已知序列都没有显着相似性。此外，我们将1173个转录本与来自C. japonica的ESTs研究组织特异性转录的可能性，发现882不存在于其他组织，如树皮和幼苗。在本研究中开发的EST很可能包括组织和阶段特异性转录本，这些将有助于进一步了解C.日本。
: We obtained 1210 expressed sequence tags (ESTs), representing 1173 transcripts, from two cDNA libraries, which derive from developing seed and pollen cones of sugi (Cryptomeria japonica). Among these 1173 transcripts, 807 showed notable similarity to known sequences, including those of genes expressed in developing flower tissues of other plant species, such as CONSTANS and the genes for MADS-domain proteins. This result indicates that, in spite of differences in morphology, the development of reproductive organs is regulated by a family of homologous transcription factors common to both gymnosperm and angiosperm species. The remaining 366 transcripts showed no significant similarity to any known sequences in public databases. Furthermore, we compared the 1173 transcripts to those derived from other tissues of C. japonica to investigate the possibility of tissue-specific transcripts, and found that 882 did not occur among ESTs derived from other tissues, such as the inner bark and seedlings. The ESTs developed during the present study most likely include tissue- and stage-specific transcripts, and these will contribute to a further understanding of the reproductive organ development of C. japonica.
影响因子：
11.6
作者：
Yano, M;Katayose, Y;Sasaki, T
通讯作者：
Sasaki, T
从未成熟花蕾中生成 919 个表达序列标签，并使用模式植物百脉根中的表达序列标签进行基因表达分析
DOI：
--
发表时间：
2002
期刊：
Genes Genet.Syst. 77
影响因子：
--
作者：
栗飯原典央 ほか;Makoto Endo 他 8名
通讯作者：
Makoto Endo 他 8名