The v-rel oncogene product is complexed with cellular proteins including its proto-oncogene product and heat shock protein 70.
The v-rel oncogene product is complexed with cellular proteins including its proto-oncogene product and heat shock protein 70.
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v-rel 癌基因产物与细胞蛋白复合，包括其原癌基因产物和热休克蛋白 70。
DOI：
10.1016/0042-6822(90)90195-w
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
BoseJr,HR
中科院分区：
文献类型：
--
作者：
Lim,MY;Davis,N;Zhang,JY;BoseJr,HR
禽网状内皮组织增生病病毒（REV-T）的癌基因产物pp 59 v-relof在REV-T转化的淋巴样细胞的胞质溶胶中与细胞蛋白复合。针对pp 59 v-rel的不同区域的单克隆抗体和抗血清除了pp 59 v-rel之外还免疫沉淀五种细胞蛋白质（p124、p115、p75、p70和p40）。具有相同表观分子量的细胞蛋白质也与pp 59 v-relduring连续Sephacryl S200和免疫亲和层析共纯化。针对复合物中最丰富的细胞蛋白pp 40的抗血清与pp 59和几种具有相同表观分子量的细胞蛋白共免疫沉淀。pp 59 v-rel复合物中的75-kDa蛋白是c-rel原癌基因的产物，并且是弱磷酸化的。在MSB-1细胞中，该蛋白质未被可检测地磷酸化或与细胞蛋白质缔合。pp 59 v-relcontaining胞质复合物中的70-kDa蛋白是禽热休克蛋白70（HSC 70）的组成形式。p70蛋白与pp 59 v相关抗血清共免疫沉淀和共纯化，抗pp 59 v相关抗血清与pp 40特异性抗血清共免疫沉淀。从含有pp 59 v的免疫复合物中分离的p70与HSC 70共享V8蛋白酶片段。
The oncogene product, pp59v-relof avian reticuloendotheliosis virus (REV-T) is complexed in the cytosol of REV-T transformed lymphoid cells with cellular proteins. Monoclonal antibodies and antisera directed against different regions of pp59v-relcoimmunoprecipitate five cellular proteins (pl24, pl 15, p75, p70, and p40) in addition to pp59v-rel. Cellular proteins with the same apparent molecular mass also copurify with pp59v-relduring sequential Sephacryl S200 and immunoaffinity chromatography. Antisera directed against the most abundant cellular protein in the complex, pp40, coimmunoprecipitate pp59v-reland several cellular proteins with the same apparent molecular mass. The 75-kDa protein in the pp59v-relcomplex is the product of c-relproto-oncogene and is weakly phosphorylated. In MSB-1 cells this protein is not detectably phosphorylated or associated with cellular proteins. The 70-kDa protein in the pp59v-relcontaining cytosolic complex is the constitutive form of avian heat shock protein 70 (HSC70). The p70 protein coimmunoprecipitates and copurifies with pp59v-relusing antisera directed against pp59v-reland coimmunoprecipitates with antisera specific for pp40. The p70 isolated from immune complexes containing pp59v-relshares V8 protease fragments with HSC70.
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DOI：
10.1073/pnas.80.20.6229
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
STEPHENS, RM;RICE, NR;GILDEN, RV
通讯作者：
GILDEN, RV
c-rel 和 c-myc 原癌基因在禽类组织中的表达。
影响因子：
8
作者：
Moore,BE;BoseJr,HR
通讯作者：
BoseJr,HR
v-rel 癌基因产物在转化的淋巴细胞中与 40-kDa 磷蛋白复合。
DOI：
10.1073/pnas.85.8.2479
发表时间：
1988
影响因子：
11.1
作者：
Tung,HY;Bargmann,WJ;Lim,MY;BoseJr,HR
通讯作者：
BoseJr,HR
网状内皮增生病毒 T 株体外转化鸡脾细胞的形态学、免疫学和生化分析。
影响因子：
11.2
作者：
T. Shibuya;I. Chen;A. Howatson;T. W. Mak
通讯作者：
T. W. Mak
DOI：
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发表时间：
--
期刊：
影响因子：
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