Identification of Aedes aegypti Long Intergenic Non-coding RNAs and Their Association with Wolbachia and Dengue Virus Infection.
Identification of Aedes aegypti Long Intergenic Non-coding RNAs and Their Association with Wolbachia and Dengue Virus Infection.
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DOI：
10.1371/journal.pntd.0005069
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发表时间：
2016-10
影响因子：
3.8
通讯作者：
Asgari S
中科院分区：
文献类型：
--
作者：
Etebari K;Asad S;Zhang G;Asgari S
长基因间非编码rna （Long intergenic non-coding rna, lincRNAs）是一类具有多种生物学功能的重要调控rna。本研究的目的是鉴定登革热媒介埃及伊蚊的lincRNA谱，并评估其在宿主-病原体相互作用中的潜在作用。以往的大多数Ae - seq转录组研究。埃及伊蚊关注的是不同生物学条件下注释蛋白编码基因的表达模式。在这里，我们使用了35个公开的RNA-Seq数据集，这些数据集的深度相对较高。发现lincRNA的埃及伊蚊基因组这导致鉴定出3,482个推测的lincrna。与蛋白编码基因相比，这些lincRNA基因的GC含量略低，转录物长度较短。Ae。即使在近亲如致倦库蚊和冈比亚按蚊中，埃及伊蚊的lincrna也表现出较低的进化序列保守性。我们检测了它们在登革热病毒2型（DENV-2）、沃尔巴克氏体感染和未感染的成蚊和Aa20细胞中的表达。结果显示，DENV-2感染增加了许多宿主lincrna的丰度，其中一些抑制了蚊子细胞中的病毒复制。rnai介导的lincRNA_1317沉默导致病毒复制增强，这可能表明它可能参与宿主抗病毒防御。许多lincrna在沃尔巴克氏体感染的蚊子中也有差异表达。该结果将有助于进一步研究lncrna在昆虫中的功能，并可能有助于开发新的方法来控制载体或抑制病毒在昆虫中的复制。埃及伊蚊是登革热和寨卡病毒等几种病毒的主要传播媒介。了解病毒与蚊子载体的复杂相互作用以及病毒复制的相关因素对于制定有效的虫媒病毒控制策略至关重要。在这项研究中，我们报告了一个由伊蚊基因组编码的长基因间非编码rna的综合列表。第一次发现埃及伊蚊。此外，我们发现许多这些长链非编码rna在感染登革热病毒的蚊子中表达差异，这可能参与了登革热病毒与蚊子的相互作用。这些结果为探索蚊子生物学和蚊子-病毒相互作用提供了一条新的途径，可能会导致发现可能有利于载体操纵的分子。
Long intergenic non-coding RNAs (lincRNAs) are appearing as an important class of regulatory RNAs with a variety of biological functions. The aim of this study was to identify the lincRNA profile in the dengue vector Aedes aegypti and evaluate their potential role in host-pathogen interaction. The majority of previous RNA-Seq transcriptome studies in Ae. aegypti have focused on the expression pattern of annotated protein coding genes under different biological conditions. Here, we used 35 publically available RNA-Seq datasets with relatively high depth to screen the Ae. aegypti genome for lincRNA discovery. This led to the identification of 3,482 putative lincRNAs. These lincRNA genes displayed a slightly lower GC content and shorter transcript lengths compared to protein-encoding genes. Ae. aegypti lincRNAs also demonstrate low evolutionary sequence conservation even among closely related species such as Culex quinquefasciatus and Anopheles gambiae. We examined their expression in dengue virus serotype 2 (DENV-2) and Wolbachia infected and non-infected adult mosquitoes and Aa20 cells. The results revealed that DENV-2 infection increased the abundance of a number of host lincRNAs, from which some suppress viral replication in mosquito cells. RNAi-mediated silencing of lincRNA_1317 led to enhancement in viral replication, which possibly indicates its potential involvement in the host anti-viral defense. A number of lincRNAs were also differentially expressed in Wolbachia-infected mosquitoes. The results will facilitate future studies to unravel the function of lncRNAs in insects and may prove to be beneficial in developing new ways to control vectors or inhibit replication of viruses in them. Aedes aegypti is a major vector of several viruses such as dengue and Zika viruses. Understanding the intricate interaction of viruses with mosquito vectors and the factors involved in virus replication are essential for developing effective arbovirus control strategies. In this study, we report a comprehensive list of long intergenic non-coding RNAs encoded by the genome of Ae. aegypti for the first time. In addition, we show that a number of these long non-coding RNAs are differentially expressed in mosquitoes infected with dengue virus, which could be involved in DENV-mosquito interaction. The outcomes provide a new avenue to explore mosquito biology and mosquito-virus interactions that may lead to the discovery of molecules that could be beneficial for vector manipulation.
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影响因子：
11.1
作者：
Bonasio R;Shiekhattar R
通讯作者：
Shiekhattar R
影响因子：
14.9
作者：
Hussain M;Torres S;Schnettler E;Funk A;Grundhoff A;Pijlman GP;Khromykh AA;Asgari S
通讯作者：
Asgari S
影响因子：
2.6
作者：
Campbell CL;Harrison T;Hess AM;Ebel GD
通讯作者：
Ebel GD
DOI：
10.1126/science.1240925
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Carpenter S;Aiello D;Atianand MK;Ricci EP;Gandhi P;Hall LL;Byron M;Monks B;Henry-Bezy M;Lawrence JB;O'Neill LA;Moore MJ;Caffrey DR;Fitzgerald KA
通讯作者：
Fitzgerald KA
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.