Generation of human blastocyst-like structures from pluripotent stem cells.
Generation of human blastocyst-like structures from pluripotent stem cells.
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从多能干细胞生成人类囊胚样结构
DOI：
10.1038/s41421-021-00316-8
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发表时间：
2021-09-07
期刊：
影响因子：
33.5
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Fan Y;Min Z;Alsolami S;Ma Z;Zhang E;Chen W;Zhong K;Pei W;Kang X;Zhang P;Wang Y;Zhang Y;Zhan L;Zhu H;An C;Li R;Qiao J;Tan T;Li M;Yu Y
人类囊胚由胚胎的前三个细胞谱系组成：滋养外胚层、上胚层和原始内胚层，所有这些对于早期发育和器官形成都至关重要。然而，由于伦理问题和人类囊胚的限制，对早期人类胚胎发生的全面了解仍然缺乏。为了弥合这一知识差距，需要一个可靠的模型系统，重现人类胚胎发生的早期阶段。在这里，我们开发了一种三维（3D），两步诱导方案，用于从人扩展多能干细胞（EPS）中产生胚泡样结构（EPS-胚状体）。形态学和单细胞转录组学分析显示，EPS-胚状体包含关键细胞谱系，并且在转录上与人类胚泡相似。此外，在胚胎结构、细胞谱系和转录谱方面，EPS-类胚体与体外培养8或10天的人胚胎相似。总之，我们开发了一个可扩展的系统来模拟人类囊胚发育，这可能有助于研究早期发育缺陷导致的早期植入失败。
Human blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast and primitive endoderm, all of which are essential for early development and organ formation. However, due to ethical concerns and restricted access to human blastocysts, a comprehensive understanding of early human embryogenesis is still lacking. To bridge this knowledge gap, a reliable model system that recapitulates early stages of human embryogenesis is needed. Here we developed a three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids are similar with human embryos that were cultured for 8 or 10 days in vitro, in terms of embryonic structures, cell lineages and transcriptomic profiles. In conclusion, we developed a scalable system to mimic human blastocyst development, which can potentially facilitate the study of early implantation failure that induced by developmental defects at early stage.
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影响因子：
64.5
作者：
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通讯作者：
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Shao Y;Taniguchi K;Gurdziel K;Townshend RF;Xue X;Yong KMA;Sang J;Spence JR;Gumucio DL;Fu J
通讯作者：
Fu J