Endocytic routes to the Golgi apparatus
Endocytic routes to the Golgi apparatus
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高尔基体的内吞途径
DOI：
--
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发表时间：
1998
影响因子：
2.3
通讯作者：
J. Roth
中科院分区：
文献类型：
--
作者：
M. Pavelka;A. Ellinger;P. Debbage;C. Loewe;M. Vetterlein;J. Roth
摘要 研究了标记凝集素和阳离子铁蛋白在分泌受调控的细胞(如胰岛β细胞)和构成分泌细胞(如成纤维细胞和肝癌细胞)中的内吞途径，特别是进入高尔基体的途径。在所有三种细胞类型中，相当数量的内化分子被摄取到跨高尔基网络(TGN)和高尔基亚室，以及胰岛β细胞的分泌颗粒中。内化的物质没有迅速通过TGN和高尔基堆栈，但在内化几个小时后仍然存在，然后特别集中在TGN元素和最前面的高尔基池中。内吞的物质到达TGN内形成分泌颗粒。此外，还观察到内吞小泡与成熟分泌颗粒的直接融合。高尔基体亚室以及含有内吞物质的内吞TGN与内质网的特化区域非常接近。高尔基体及其含有内吞物质的部分在真菌代谢物Brefeldin A处理后转变为管状网，内质网的管腔内很少观察到内化的物质，从而为内质膜至内质网的途径提供了证据。
Abstract The endocytic routes of labelled lectins as well as cationic ferritin were studied in cells with a regulated secretion, i.e. pancreatic beta cells, and in constitutively secreting cells, i.e. fibroblasts and HepG2 hepatoma cells, paying particular attention to routes into the Golgi apparatus. Considerable amounts of internalised molecules were taken up into the trans Golgi network (TGN) and into Golgi subcompartments in all three cell types as well as in secretory granules of the pancreatic beta cells. The internalised materials did not pass rapidly the TGN and Golgi stacks, but were still present hours after internalisation, being then particularly concentrated in TGN-elements and in the transmost Golgi cisterna. Endocytosed materials reached forming secretory granules present in the TGN. Further, direct fusion between endocytotic vesicles and mature secretory granules was observed. Golgi subcompartments as well as endocytic TGN containing endocytosed materials were in close apposition to specialised regions of the endoplasmic reticulum. The Golgi apparatus including its parts containing endocytosed materials were transformed into a tubular reticulum upon treatment with the fungal metabolite Brefeldin A. Rarely, internalised material was observed in the lumen of the endoplasmic reticulum, thus providing evidence for an endocytic plasma membrane to endoplasmic reticulum route.
在培养的人体细胞中将表面甘露糖 6-磷酸受体转运至高尔基复合体。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jin,M;SahagianJr,GG;Snider,MD
通讯作者：
Snider,MD
在受调节的分泌细胞系中，内吞膜运输至高尔基体。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Green,SA;Kelly,RB
通讯作者：
Kelly,RB
分泌细胞中的膜回收：通往高尔基复合体的途径。
DOI：
10.1002/9780470720745.ch9
发表时间：
1982
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
Farquhar,MG
通讯作者：
Farquhar,MG