Perkinsosis in molluscs: A review
Perkinsosis in molluscs: A review
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软体动物帕金森病：综述
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
A. Figueras
中科院分区：
文献类型：
--
作者：
A. Villalba;K. Reece;M. Ordás;S. Casas;A. Figueras
Perkinsus属包括感染全世界海洋软体动物的原生生物寄生虫，其中一些与大规模死亡有关。生活史包括在宿主体内的营养增殖，通过营养增殖，一个称为滋养体的细胞经历连续的二次分配。其他阶段已观察到在体外或体内，这取决于物种：休眠孢子，游动孢子囊和游动孢子。分子分类学支持甲藻和帕金藻之间的密切关系。目前有六种帕金虫被认为是有效的：P. marinus、P. olseni、P. qugwadi、P. chesapeaki、P. andrewsi和P. meditalieus。组织学，尤其是宿主组织在雷氏硫乙醇酸盐流体培养基（RFTM）中的孵育是经典的诊断方法。此外，更敏感和更快的分子诊断技术的基础上，无论是免疫测定或PCR已开发的珀金虫属。流行动物学研究表明，水温和盐度对海滨牡蛎中的海洋疫霉感染有显著影响，从而确定了寄生虫的地理范围和时间疾病动态（季节性）。在体外培养已经建立了四个珀金虫属。对感染的免疫应答根据宿主而变化，并且涉及宿主血细胞对寄生虫细胞的吞噬作用或包囊作用。菲律宾蛤蚌血细胞分泌的一种多肽可以杀死寄生虫。在体外培养的海洋原卟啉单胞菌细胞分泌蛋白酶，这些蛋白酶可能参与宿主组织的降解。海洋原甲藻能抑制宿主血细胞产生的有毒氧自由基。除了宿主死亡外，帕金虫属引起的亚致死效应也是一个重要的原因。（繁殖力、生长和状况的降低）可能具有显著的生态和经济影响。已经分析了各种策略来减轻P. marinus流行病对牡蛎产业的影响：管理/养殖程序的修改，选择性育种以获得抗性牡蛎品系，以及使用三倍体牡蛎和外来牡蛎物种。一些化疗药物已被证明在体外抑制或杀死寄生虫细胞。
The genus Perkinsus includes protistan parasites infecting marine molluscs throughout the world, some of which are associated with mass mortalities. Life cycle involves vegetative proliferation within the host, by which a cell named trophozoite undergoes successive bipartitioning. Other stages have been observed in vitro or in vivo, depending on the species: hypnospore, zoosporangium and zoospore. Molecular taxonomy supports a close affinity be- tween dinoflagellates and Perkinsus spp. Six species of Perkinsus are currently considered valid: P. marinus, P. olseni, P. qugwadi, P. chesapeaki, P. andrewsi and P. mediterraneus. Histology and, above all, incubation of host tissues in Ray's fluid thioglycollate medium (RFTM) are classic diagnostic methods. In addition, more sensitive and quicker molecular diagnostic techniques based on either immunoassays or PCR have been developed for Perkinsus spp. Epi- zootiological studies have shown a marked influence of water temperature and salinity on P. marinus infection in oys- ters Crassostrea virginica, thus determining parasite geographical range and temporal disease dynamics (seasonality). In vitro cultures have been established for four Perkinsus spp. Immune response to infection varies depending on host and involves phagocytosis or encapsulation of the parasite cells by host haemocytes. A polypeptide is secreted by clam Tapes philippinarum haemocytes that could kill the parasite. In vitro cultured P. marinus cells secrete proteases that are likely involved in degradation of host tissues. P. marinus can suppress the toxic oxygen radicals produced by host haemocytes. In addition to host death, sublethal effects caused by Perkinsus spp. (reduction of fecundity, growth, and condition) may have significant ecological and economic implications. Various strategies have been assayed to mitigate the consequences of P. marinus epizootics on the oyster industry: modifications of management/culture procedures, selective breeding to obtain resistant oyster strains, and the use of triploid oysters and allochthonous oyster species. Some chemotherapeutants have been proved to inhibit or kill parasite cells in vitro.
利什曼原虫磷酸酶可阻断中性粒细胞 O-2 的产生。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Remaley,AT;Kuhns,DB;Basford,RE;Glew,RH;Kaplan,SS
通讯作者：
Kaplan,SS
软体动物血浆中的蛋白酶抑制活性：光滑双脐贝中存在 α-巨球蛋白的证据。
DOI：
10.1016/0305-0491(92)90086-7
发表时间：
1992
期刊：
Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子：
--
作者：
Bender,RC;Fryer,SE;Bayne,CJ
通讯作者：
Bayne,CJ
DOI：
10.1073/pnas.92.13.5793
发表时间：
1995-06-20
影响因子：
11.1
作者：
ESCALANTE, AA;AYALA, FJ
通讯作者：
AYALA, FJ