Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: implications for evolution of arenaviruses in Africa.
Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: implications for evolution of arenaviruses in Africa.
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DOI：
10.1371/journal.pone.0020893
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Günther S
中科院分区：
文献类型：
--
作者：
Coulibaly-N'Golo D;Allali B;Kouassi SK;Fichet-Calvet E;Becker-Ziaja B;Rieger T;Olschläger S;Dosso H;Denys C;Ter Meulen J;Akoua-Koffi C;Günther S
这项研究旨在识别新的沙粒病毒，并收集非洲沙粒病毒进化的见解。2003年至2005年期间，在科特迪瓦南部、东部和中西部的9个村庄捕获了14个不同属的1 228只小型哺乳动物。标本通过靶向S和L RNA片段的泛旧世界沙粒病毒RT-PCR以及免疫荧光试验筛选。在毛鼠和小家鼠体内分别检测到沙粒病毒科的两个新的暂定种Menekre和Gbagroube病毒的序列。血清学试验也证实了沙鼠的沙粒病毒感染。没有发现拉沙病毒，尽管60%的捕获动物是Mastomys natalensis。从啮齿动物标本中回收了新病毒的完整S RNA和部分L RNA序列，并进行了系统发育分析。Gbagroube病毒是拉沙病毒的一个密切相关的姐妹分类群，而Menekre病毒则与Ippy/Mobala/Mopeia病毒复合体聚在一起。重建可能的病毒-宿主共进化情景表明，在非洲大陆，共同进化的特征可能已被多个宿主转换事件所消除。
This study aimed to identify new arenaviruses and gather insights in the evolution of arenaviruses in Africa. During 2003 through 2005, 1,228 small mammals representing 14 different genera were trapped in 9 villages in south, east, and middle west of Côte d'Ivoire. Specimens were screened by pan-Old World arenavirus RT-PCRs targeting S and L RNA segments as well as immunofluorescence assay. Sequences of two novel tentative species of the family Arenaviridae, Menekre and Gbagroube virus, were detected in Hylomyscus sp. and Mus (Nannomys) setulosus, respectively. Arenavirus infection of Mus (Nannomys) setulosus was also demonstrated by serological testing. Lassa virus was not found, although 60% of the captured animals were Mastomys natalensis. Complete S RNA and partial L RNA sequences of the novel viruses were recovered from the rodent specimens and subjected to phylogenetic analysis. Gbagroube virus is a closely related sister taxon of Lassa virus, while Menekre virus clusters with the Ippy/Mobala/Mopeia virus complex. Reconstruction of possible virus–host co-phylogeny scenarios suggests that, within the African continent, signatures of co-evolution might have been obliterated by multiple host-switching events.
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影响因子：
10.7
作者：
Hughes, AL;Friedman, R
通讯作者：
Friedman, R
影响因子：
9.4
作者：
Drosten, C;Göttig, S;Günther, S
通讯作者：
Günther, S
影响因子：
3.8
作者：
Fichet-Calvet, Elisabeth;Rogers, David John
通讯作者：
Rogers, David John
影响因子：
9.8
作者：
Drummond AJ;Ho SY;Phillips MJ;Rambaut A
通讯作者：
Rambaut A
影响因子：
11.8
作者：
Günther S;Hoofd G;Charrel R;Röser C;Becker-Ziaja B;Lloyd G;Sabuni C;Verhagen R;van der Groen G;Kennis J;Katakweba A;Machang'u R;Makundi R;Leirs H
通讯作者：
Leirs H