High Incidence of Herpes Simplex Virus-1 in Cord Blood and Placenta Infection of Women in Southern Brazil.
High Incidence of Herpes Simplex Virus-1 in Cord Blood and Placenta Infection of Women in Southern Brazil.
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DOI：
10.1055/s-0039-1700794
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发表时间：
2020-01
影响因子：
1.2
通讯作者：
Barral de Martinez, Ana Maria
中科院分区：
文献类型：
--
作者：
Avila, Emiliana Claro;Finger-Jardim, Fabiana;Goncalves, Carla Vitola;da Hora, Vanusa Pousada;Soares, Marcelo Alves;Barral de Martinez, Ana Maria
目的了解人类疱疹病毒1型（HSV-1）DNA在胎盘组织、新生儿脐带血中的感染率及其相关危险因素。 方法对某大学医院480例无症状产妇及其新生儿的胎盘活检和脐带血进行分析。 巢式聚合酶链反应（PCR）和基因测序用于识别病毒;比值比（OR）和相对危险度（RR）进行比较与这种情况相关的危险因素。结果胎盘组织中HSV-1DNA的检出率为37.5%，脐带血中HSV-1DNA的检出率为27.5%。 在61.4%的HSV-1+脐带血与胎盘组织配对样本中确定了血源性经胎盘途径。在其余38.6%的胎盘组织中没有观察到病毒的证据，表明来自生殖道的上升感染，在胎盘组织中没有复制，导致羊膜内感染和垂直传播，通过脐带血中的病毒观察到。不使用避孕套会增加在胎盘和脐带血中发现HSV-1的风险。结论胎盘和脐血中均检出HSV-1DNA，提示HSV-1DNA可由无症状孕妇经母婴垂直传播。
Objective Estimate the prevalence of human herpesvirus type 1 HSV-1 DNA in placental samples, its incidence in umbilical cord blood of newborns and the associated risk factors. Methods Placental biopsies and umbilical cord blood were analyzed, totaling 480 samples, from asymptomatic parturients and their newborns at a University Hospital. Nested polymerase chain reaction (PCR) and gene sequencing were used to identify the virus; odds ratio (OR) and relative risk (RR) were performed to compare risk factors associated with this condition. Results The prevalence of HSV-1 DNA in placental samples was 37.5%, and the incidence in cord blood was 27.5%. Hematogenous transplacental route was identified in 61.4% from HSV-1+ samples of umbilical cord blood paired with the placental tissue. No evidence of the virus was observed in the remaining 38.6% of placental tissues, suggesting an ascendant infection from the genital tract, without replication in the placental tissue, resulting in intra-amniotic infection and vertical transmission, seen by the virus in the cord blood. The lack of condom use increased the risk of finding HSV-1 in the placenta and umbilical cord blood. Conclusion The occurrence of HSV-1 DNA in the placenta and in cord blood found suggests vertical transmission from asymptomatic pregnant women to the fetus.
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