Local tumor control and DNA-PK activity of peripheral blood lymphocytes in prostate cancer patients receiving radiotherapy.
Local tumor control and DNA-PK activity of peripheral blood lymphocytes in prostate cancer patients receiving radiotherapy.
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前列腺癌患者的外周血淋巴细胞的局部肿瘤控制和DNA-PK活性接受放疗。
DOI：
10.1093/jrr/rrw099
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发表时间：
2017-03-01
影响因子：
2
通讯作者：
Sakata KI
中科院分区：
文献类型：
--
作者：
Someya M;Hasegawa T;Hori M;Matsumoto Y;Nakata K;Masumori N;Sakata KI
DNA损伤的修复对于基因组的稳定性至关重要，而DNA依赖性蛋白激酶（DNA-PK）在修复双链断裂中具有重要作用。我们研究了接受放射治疗的前列腺癌患者外周血淋巴细胞（PBL）的DNA-PK活性是否与生化（前列腺特异性抗原：PSA）复发和放射毒性相关。共有69例局限性前列腺癌患者参加了本研究。放疗后2年或2年后采集外周血，离心，然后通过滤膜结合试验测量DNA-PK活性。高DNA-PK活性组的PSA无复发生存率显著高于低DNA-PK活性组。高DNA-PK活性组的10年PSA无复发生存率为87.0%，而低DNA-PK活性组为52.7%。多因素分析显示Gleason评分和DNA-PK活性水平是PSA放疗后复发的显著预测因素。此外，低DNA-PK活性组的1-2级尿毒性发生率往往高于高DNA-PK活性组。DNA-PK活性低的前列腺癌患者PSA复发率和尿毒性发生率较高。PBLs中的DNA-PK活性可能是预测PSA复发和尿毒性的有用标志物，可能有助于前列腺癌的个体化治疗。
Repair of DNA damage is critical for genomic stability, and DNA-dependent protein kinase (DNA-PK) has an important role in repairing double-strand breaks. We examined whether the DNA-PK activity of peripheral blood lymphocytes (PBLs) was related to biochemical (prostate-specific antigen: PSA) relapse and radiation toxicity in prostate cancer patients who have received radiotherapy. A total of 69 patients with localized adenocarcinoma of the prostate participated in this study. Peripheral blood was collected 2 years or later after radiotherapy and centrifuged, then DNA-PK activity was measured by a filter binding assay. The high DNA-PK activity group had a significantly higher PSA relapse–free survival rate than the low DNA-PK activity group. The 10-year PSA relapse–free survival was 87.0% in the high DNA-PK activity group, whereas it was 52.7% in the low DNA-PK activity group. Multivariate analysis showed the Gleason score and the level of DNA-PK activity were significant predictors of PSA relapse after radiotherapy. In addition, the low DNA-PK activity group tended to have a higher incidence of Grade 1–2 urinary toxicity than the high DNA-PK activity group. Prostate cancer patients with low DNA-PK activity had a higher rate of PSA relapse and a higher incidence of urinary toxicity. DNA-PK activity in PBLs might be a useful marker for predicting PSA relapse and urinary toxicity, possibly contributing to personalized treatment of prostate cancer.
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DOI：
10.1042/bj20080413
发表时间：
2009-02-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Mahaney BL;Meek K;Lees-Miller SP
通讯作者：
Lees-Miller SP
影响因子：
2
作者：
Yamazaki H;Nakamura S;Nishimura T;Yoshida K;Yoshioka Y;Koizumi M;Ogawa K
通讯作者：
Ogawa K
影响因子：
2
作者：
Sharma MK;Imamichi S;Fukuchi M;Samarth RM;Tomita M;Matsumoto Y
通讯作者：
Matsumoto Y
影响因子：
8.8
作者：
Someya, M.;Sakata, K-I;Matsumoto, Y.;Kamdar, R. P.;Kai, M.;Toyota, M.;Hareyama, M.
通讯作者：
Hareyama, M.
影响因子：
--
作者：
Berlin A;Lalonde E;Sykes J;Zafarana G;Chu KC;Ramnarine VR;Ishkanian A;Sendorek DH;Pasic I;Lam WL;Jurisica I;van der Kwast T;Milosevic M;Boutros PC;Bristow RG
通讯作者：
Bristow RG