The association of DNA-dependent protein kinase activity of peripheral blood lymphocytes with prognosis of cancer.
The association of DNA-dependent protein kinase activity of peripheral blood lymphocytes with prognosis of cancer.
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DOI：
10.1038/bjc.2011.158
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发表时间：
2011-05-24
影响因子：
8.8
通讯作者：
Hareyama, M.
中科院分区：
文献类型：
--
作者：
Someya, M.;Sakata, K-I;Matsumoto, Y.;Kamdar, R. P.;Kai, M.;Toyota, M.;Hareyama, M.
关键词：
修复各种类型的DNA损伤对基因组的稳定性至关重要。DNA依赖性蛋白激酶（DNA- pk）在DNA双链断裂修复中起着重要作用。我们研究了不同癌症患者外周血淋巴细胞（pbl）中DNA-PK活性与生存率之间是否存在相关性。我们还研究了放射治疗后pbl中DNA-PK活性的变化。共有167名未经治疗的癌症患者参与了这项研究。收集外周血，分离，离心。采用dna下拉法测定DNA-PK活性。用细胞遗传学方法检测染色体畸变。晚期肿瘤患者pbl的DNA-PK活性明显低于早期。pbl中DNA-PK活性较低的患者在晚期比DNA-PK活性较高的患者有更低的疾病特异性生存和远端无转移生存。DNA-PK活性与多余片段呈负相关。大多数患者pbl的DNA-PK活性随着等效全身剂量的增加而降低。晚期肿瘤患者，pbl DNA-PK活性较低，远处转移率较高，预后较差。因此，pbl中DNA-PK活性可作为预测染色体不稳定性和预后不良的标志物。
Repair of various types of DNA damages is critical for genomic stability. DNA-dependent protein kinase (DNA-PK) has an important role in DNA double-strand break repair. We examined whether there may be a correlation between DNA-PK activity in peripheral blood lymphocytes (PBLs) and survival percentages in various cancer patients. We also investigated the changes of DNA-PK activity in PBLs after radiotherapy. A total of 167 of untreated cancer patients participated in this study. Peripheral blood was collected, separated, and centrifuged. DNA-PK activity was measured by DNA-pull-down assay. Chromosomal aberrations were examined by cytogenetic methods. DNA-PK activity of PBLs in advanced cancer patients was significantly lower than that in early stage. The patients with lower DNA-PK activity in PBLs tended to have the lower disease-specific survivals and distant metastasis-free survivals than those with higher DNA-PK activity in advanced stages. There was also a tendency of inverse correlation between DNA-PK activity and excess fragments. The DNA-PK activity of PBLs in most patients decreased in response to radiation as the equivalent whole-body dose increased. Cancer patients in advanced stage, with lower DNA-PK activity of PBLs might have higher distant metastasis and exhibit poorer prognosis. Therefore, DNA-PK activity in PBLs could be used as a marker to predict the chromosomal instability and poorer prognosis.
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DOI：
10.1016/0360-3016(93)90345-v
发表时间：
1993-01-15
影响因子：
7
作者：
TAGHIAN, A;RAMSAY, J;SUIT, H
通讯作者：
SUIT, H
影响因子：
2.6
作者：
THIERENS, H;VRAL, A;DERIDDER, L
通讯作者：
DERIDDER, L
影响因子：
5.3
作者：
LEESMILLER, SP;SAKAGUCHI, K;ANDERSON, CW
通讯作者：
ANDERSON, CW
影响因子：
30.8
作者：
Chin, K;de Solorzano, CO;Lockett, SJ
通讯作者：
Lockett, SJ
DOI：
10.1139/o96-054
发表时间：
1996-01-01
期刊：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
影响因子：
--
作者：
LeesMiller, SP
通讯作者：
LeesMiller, SP