Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
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草鱼 AlaSerCys 转运蛋白 2 的分子特征和饮食调节
DOI：
10.1111/jwas.12339
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发表时间：
2017-04
影响因子：
2.8
通讯作者：
Guo Xinhong
中科院分区：
文献类型：
--
作者：
Hu Rong;Wang Yonghong;Qu Fufa;Tang Jianzhou;Zhou Yi;Lu Shuangqing;Zhang Jianshe;Liu Zhen;Zhou Zhigang;Guo Xinhong
AlaSerCys Transporter 2 (ASCT2), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full-length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella, which encodes a 541-amino-acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio. ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real-time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2. These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.
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影响因子：
3.1
作者：
V. Torres-zamorano;F. Leibach;V. Ganapathy
通讯作者：
V. Torres-zamorano;F. Leibach;V. Ganapathy
日粮添加N-氨甲酰谷氨酸可增加断奶环江小型猪仔猪肠道氨基酸转运蛋白的表达
影响因子：
3.3
作者：
Yang, H. S.;Fu, D. Z.;Yin, Y. L.
通讯作者：
Yin, Y. L.
影响因子：
2.9
作者：
Juan Tian;G. He;K. Mai;Chengdong Liu
通讯作者：
Juan Tian;G. He;K. Mai;Chengdong Liu
影响因子：
2.4
作者：
STEVENS, BR;ROSS, HJ;WRIGHT, EM
通讯作者：
WRIGHT, EM
DOI：
10.1111/j.1753-4887.1990.tb02968.x
发表时间：
1974-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. G. Windmueller;A. Spaeth
通讯作者：
H. G. Windmueller;A. Spaeth