Compensation for intracellular environment in expression levels of mammalian circadian clock genes.
Compensation for intracellular environment in expression levels of mammalian circadian clock genes.
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DOI：
10.1038/srep04032
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发表时间：
2014-02-07
影响因子：
4.6
通讯作者：
Akashi M
中科院分区：
文献类型：
--
作者：
Matsumura R;Okamoto A;Node K;Akashi M
几乎所有体细胞中的生物钟都是由生物钟基因的转录振荡驱动的。为了研究细胞类型特异性细胞内环境对昼夜节律机制的影响，我们检测了五种外周组织的基因表达谱。正如预期的那样，9个时钟基因的表达节律之间的相位关系在所有被检测的组织中都是相似的。我们还比较了生物钟基因在组织中的相对表达水平，意外地发现，其数量变化保持在大约三倍的范围内，这大大小于代谢管家基因。有趣的是，即使用不同浓度的血清培养成纤维细胞，昼夜节律基因表达也几乎没有受到影响。总之，这些发现支持了一个假设，即时钟基因的表达水平在细胞内环境（如氧化还原电位和代谢物组成）的数量上得到补偿。然而，需要更全面的研究才能得出明确的结论。
The circadian clock is driven by transcriptional oscillation of clock genes in almost all body cells. To investigate the effect of cell type-specific intracellular environment on the circadian machinery, we examined gene expression profiles in five peripheral tissues. As expected, the phase relationship between expression rhythms of nine clock genes was similar in all tissues examined. We also compared relative expression levels of clock genes among tissues, and unexpectedly found that quantitative variation remained within an approximately three-fold range, which was substantially smaller than that of metabolic housekeeping genes. Interestingly, circadian gene expression was little affected even when fibroblasts were cultured with different concentrations of serum. Together, these findings support a hypothesis that expression levels of clock genes are quantitatively compensated for the intracellular environment, such as redox potential and metabolite composition. However, more comprehensive studies are required to reach definitive conclusions.
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