Comparison of Direct and Indirect cold atmospheric-pressure plasma methods in the B(16)F(10) melanoma cancer cells treatment.
Comparison of Direct and Indirect cold atmospheric-pressure plasma methods in the B(16)F(10) melanoma cancer cells treatment.
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DOI：
10.1038/s41598-018-25990-9
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发表时间：
2018-05-16
影响因子：
4.6
通讯作者：
Shokri B
中科院分区：
文献类型：
--
作者：
Saadati F;Mahdikia H;Abbaszadeh HA;Abdollahifar MA;Khoramgah MS;Shokri B
在这项研究中，采用并研究了一种新方法，以便在临床水平上破坏小鼠体内的癌细胞。在体外研究中，采用MTT测定来发现应用血浆的有效剂量，并区分直接和间接治疗中的血浆效果。还在体内切片中测量了肿瘤生长，以便比较直接和间接治疗的有效性。此外，还进行了一项调查，以研究传统方法（化疗）和等离子体治疗之间的干扰，以提高体内治疗的有效性。使用苏木精和曙红、流式细胞术、TUNEL 和蛋白质印迹测定来研究任何细胞改变以及各种治疗方法对癌细胞及其凋亡量和蛋白质水平的影响。拍摄放射学和 CT 扫描图像以确定最终的肿瘤体积。结果显示，与间接等离子体治疗相比，直接等离子体治疗中的细胞显着死亡，肿瘤生长显着减少。最终，在使用间接血浆化疗组合时观察到癌细胞的显着破坏，从而引入了一种治疗深部组织肿瘤的有效方法。
In this study a novel method was implemented and investigated in order to destroy cancer cells inside the mouse body on a clinical level. In the case of in-vitro study, MTT assay was employed to discover an effective dose of applied plasma and distinguish the plasma effect in direct and in indirect treatments. Tumor growth was also measured in in-vivo section so that the effectiveness of direct and indirect treatments could be compared. Furthermore, an investigation was conducted to study the interferences between a conventional method (chemotherapy) and plasma treatment so as to increase the effectiveness of treatment inside the body. Hematoxylin and Eosin, Flow Cytometry, TUNEL and Western Blot assay were used to investigate any cell alteration and the impact of various treatment methods on cancer cell and amount of their apoptosis and protein levels. Radiology and CT scan images were taken to determine the final tumor volume. The results showed a significant cell death and substantial reduction in tumor growth in direct plasma treatment in comparison with indirect plasma treatment. Eventually, dramatic destruction of cancer cells was observed while using of indirect plasma-chemotherapy combination, thus introducing an effective method for deep tissue tumors can be introduced.
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影响因子：
3.7
作者：
Cheng X;Sherman J;Murphy W;Ratovitski E;Canady J;Keidar M
通讯作者：
Keidar M
影响因子：
3.7
作者：
Brun P;Brun P;Vono M;Venier P;Tarricone E;Deligianni V;Martines E;Zuin M;Spagnolo S;Cavazzana R;Cardin R;Castagliuolo I;Valerio AL;Leonardi A
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Leonardi A
影响因子：
5.6
作者：
Huyan, Xiao-Hui;Lin, Ying-Ping;Fan, Yi-Ming
通讯作者：
Fan, Yi-Ming
影响因子：
3.8
作者：
Bourdon, Anne;Darny, Thibault;Robert, Eric
通讯作者：
Robert, Eric
影响因子：
4.6
作者：
Judée F;Fongia C;Ducommun B;Yousfi M;Lobjois V;Merbahi N
通讯作者：
Merbahi N